The influence on the melting of calf thymus and plasmid DNA of cationic lipids of the type used in gene therapy was studied by ultraviolet spectrophotometry and differential scanning calorimetry. It was found that various membrane-forming cationic lipids are able to pro...

The molecular basis of idiopathic ventricular fibrillation (IVF) is poorly understood. Previously, we identified crucial role of DPP6, a Kv4.3-associating β-subunit, in Purkinje fiber Ito and gene variants causing DPP6 gain-of-function in IVF. This study investigated th...

The ability to discriminate current modulations produced by various nucleotides while single stranded DNA is being electrophoretically driven through a biological nanopore offers a simple and inexpensive technique for DNA sequencing. However, to realize the potential of...

AbstractConcentration is a key parameter in controlling the aggregation of self-assembling oligopeptides. By investigating the concentration effects, an aggregation mechanism of EAK16-II is proposed. Depending on the critical aggregation concentration (CAC) of EAK16-II,...

To study the initial kinetics of Ins(1,4,5)P3-induced [Ca2+]i elevations with a high time resolution and to avoid the problem of cell-to-cell heterogeneity, we have used the combined patch-clamp/microfluorimetry technique. The mathematical description of the microperfus...

We examined the hypothesis that calsequestrin (CSQ2) can regulate the RyR2 activity using a combination of single channel electrophysiology and lanthanide resonance energy transfer (LRET). Under steady-state, open probability (Po) of RyR2 of cardiac SR fractions from do...

Interactions of monomeric alpha-synuclein (alphaS) with lipid membranes have been suggested to play an important role in initiating aggregation of alphaS. We have systematically analyzed the distribution and self-assembly of monomeric alphaS on supported lipid bilayers....

The formation of self-reproducing fatty acid vesicles is believed to be a quintessential building block required for (the origin of) life. In literature a number of vesicle reproduction experiments have been reported [1]. Of particular interest is the so called matrix e...

The Voltage-Dependent Anion Channel (VDAC) controls the fluxes of ATP/ADP and other respiratory substrates across the mitochondrial outer membrane (MOM) by using its characteristic ability to switch or “gate” between high-conducting “open” and low-conducting “closed” st...

The treatment of cardiac arrhythmia is largely empirical due to incomplete understanding of antiarrhythmic drug-receptor dynamics. However, our laboratory has recently determined that drug receptor interactions of commonly prescribed local anesthetics (LAs) can be descr...