• Canada
• Research dataclose
• Research softwareclose
• Open Access close
• Canadian Institutes of Health Research close

Additional file 19: Supplementary Movie 12. Description: Time-lapse imaging of GFP-0N4R reporter cells seeded with S1 brain fractions including pathogenic tau derived from aged TgTauP301L mice with neurological signs. Images were obtained for 16 hours (10 min/frame for 96 frames). Scale bars, 10 μm.

A, Western blot showing AMY3 transfected HEK293 cells demonstrate a marked increase in level of expression of CTR and RAMP3 proteins compared to wild-type (WT) HEK cells. B, in BV2 cells, RAMP3 protein expression shows a marked decreased after RAMP3 siRNA transfection compared to the control non-transfected cells. (JPEG 1495 kb)

Complete set of 739 unique cyclodipeptide synthases identified in a global analysis of publicly available prokaryotic genomes. (XLSX 144Â kb)

Alignment-based metrics for reverse transcriptase comparisons. Table S2. Alignment-based metrics for the size selection experiment. Table S3. Alignment-based metrics for the comparison of library construction kits. Table S4. Alignment-based metrics for the intermediate ssRNA-seq pipeline. The protocol evaluated includes all changes (1st strand cDNA synthesis, optimal bead purifications, new library construction chemistry with modified ligation condition, bead-based size selection, and UNG treatment) with the exception of the mRNA isolation improvements. Table S5. Alignment-based metrics for the final ssRNA-seq pipeline using UHR. Table S6. Alignment-based metrics for the final ssRNA-seq pipeline using tumor samples. (XLS 62Â kb)

Additional file 4: Table S3. A. Results for 34 independent ARIC Discovery Meta-Analysis identified mtDNA-CN associated CpGs across all studied cohorts and Validation Meta-Analysis/All Cohort Meta-Analysis. Validation meta-analysis included CHS AA, CHS EA and FHS EA cohorts (P

Targets for goal-directed action can be encoded in allocentric coordinates (relative to another visual landmark), but it is not known how these are converted into egocentric commands for action. Here, we investigated this using a slow event-related fMRI paradigm, based on our previous behavioral finding that the Allocentric to Egocentric (Allo-Ego) conversion for reach is done at the first possible opportunity. Participants were asked to remember (and eventually reach toward) the location of a briefly presented target relative to another visual landmark. After a 1st memory delay, participants were forewarned by a verbal instruction if the landmark would reappear at the same location, (potentially allowing them to plan a reach following the auditory cue before the 2nd delay), or at a different location where they had to wait for the final landmark to be presented before response, and then reach toward the remembered target location. As predicted, participants showed landmark-centered directional selectivity in occipital-temporal cortex during the first memory delay, only developed egocentric directional selectivity in occipital-parietal cortex during the second delay for the “Same cue” task, and during response for the “Different cue” task. We then compared cortical activation between these two tasks at the times when the Allo-Ego conversion occurred, and found common activation in right precuneus, right pre-supplementary area and bilateral dorsal premotor cortex. These results confirm that the brain converts allocentric codes to egocentric plans at the first possible opportunity, and identify the four most likely candidate sites specific to the Allo-Ego transformation for reaches.

Kernel density curves of the hdPS distribution within the Full Cohort. (TIF 460Â kb)

Previously described Ď Neoblast markers are not specific to the Ď -class. Plots were made using an online single cell RNAseq resource published by [40] ( http://radiant.wi.mit.edu/app/ ). Legend of neoblast classes in top right. (PNG 245 kb)

Figure S1. The genotype of NOD1/2 DKO mice. Genomic DNA from mice was amplified by PCR with specific primers to detect the disrupted sequences on a 1% agarose gel with a 100Â bp marker. First 5 bands are the KO mice and the last bands are the WT mice in A and B. NÂ =â 5/3 per group. (TIFF 12868 kb)

Copy number variation (CNV) segments in primary and recurrence tumors from 8 of 16 pairs of pHGG with matched normal tissue available. (XLSX 71Â kb)