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Simulation of a black-hole binary system evolved by the SpEC code.

Simulation of a black-hole binary system evolved by the SpEC code.

Simulation of a black-hole binary system evolved by the SpEC code.

The correlation between gender and CSF BACE activity. (TIF 2480 kb)

In July 2017 members of the DTES community started meeting as a collective at the Hives for Humanity Bee Space to have conversation about how to ensure that community ethics are a respected part of the process of cultural production. We define cultural production as being: any time an entity comes into a community to make a product from its culture. ie. individuals and/or organisations of journalists, film makers, photographers, students, researchers, tourists or volunteers. We define community ethics as being: a set of principles to guide behaviour, based in lived experience, acknowledging the interconnectedness of our humanity, fostering relationships of respect, responsibility, reciprocity and return. We have produced a resource card and a manifesto out of these meetings which we are launching at our event on March 7th 2019, 7pm-9pm at SFU Woodwards. Copies of the card and manifesto will be available for all to take out into the community, and will be open sourced after the event. The evening included a short panel discussion with members of the collective sharing their experiences of cultural production – the good, the bad and the ugly! For more info visit: hivesforhumanity.com/communityethics/

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Simulation of a black-hole binary system evolved by the SpEC code.

Additional file 23. Microarray image data from replicate 3 of MCF-7 breast cancer cells grown in static culture.

U-2 OS cells were seeded onto 8-well LabTek II imaging chambers (ThermoFisher) in complete media with 1 μg/mL tetracycline and grown for 24 h to allow expression of GFP-tagged protein. Before imaging, the media was replaced with 200 μL of phenol red-free DMEM supplemented with 10% FBS, 1 mM sodium pyruvate, and 1× Glutamax (all Life Technologies). Images stacks were acquired on an imaging system (DeltaVision Elite, GE Healthcare). Cells were imaged at 37°C in 5% CO2 at 60×, 1.42NA, with 2×2 binning. Image Z-stacks of 24 μm were aquired at 2 μm intervals over 2 - 5 min as indicated. 20 s after the start of data acquisition, 100 μL of warm media containing 1.5 μM JQ1 was added to each cell chamber manually for a final concentration of 500 nM. The exposure time was 10 ms at 32% for GFP-tagged bait protein. Z-stacks were collected, deconvolved using softWoRx (v5.0, Applied Precision) and displayed as maximum intensity projections (pixel size 0.1075 μm). Images were cropped in ImageJ (National Institutes of Health). Supplemental Movie S1 – Flp-In T-REx U-2 OS cells expressing GFP-tagged BRD2 treated with 500 nM JQ1 for 2 min. Supplemental Movie S2 – Flp-In T-REx U-2 OS cells expressing GFP-tagged BRD3 (WT construct) treated with 500 nM JQ1 for 2 min. Supplemental Movie S3 – Flp-In T-REx U-2 OS cells expressing GFP-tagged BRD4 treated with 500 nM JQ1 for 2 min. Supplemental Movie S4 – Flp-In T-REx U-2 OS cells expressing GFP-tagged BRD3 BD1mut treated with 500 nM JQ1 for 2 min. Supplemental Movie S5 – Flp-In T-REx U-2 OS cells expressing GFP-tagged BRD3 BD2mut treated with 500 nM JQ1 for 2 min. Supplemental Movie S6 – Flp-In T-REx U-2 OS cells expressing GFP-tagged BRD3 (BD1:2)mut treated with 500 nM JQ1 for 2 min.

Simulation of a black-hole binary system evolved by the SpEC code.