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Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.

Oil crops are one of the most important agricultural commodities. In the U.K. (and Northern Europe and Canada) oilseed rape is the dominant oil crop and worldwide it accounts for about 12% of the total oil and fat production. There is an increasing demand for plant oils not only for human food and animal feed but also as renewable sources of chemicals and biofuels. This increased demand has shown a doubling every 8 years over the last four decades and is likely to continue at, at least, this rate in the future. With a limitation on agricultural land, the main way to increase production is to increase yields. This can be achieved by conventional breeding but, in the future, significant enhancements will need genetic manipulation. The latter technique will also allow specific modification of the oil product to be achieved. In order for informed genetic manipulation to take place, a thorough knowledge of the biosynthesis of plant oils is needed. Crucially, this would include how regulation of oil quality and quantity is controlled. The synthesis of storage oil in plant seeds is analogous to a factory production line, where the supply of raw materials, manufacture of components and final assembly can all potentially limit the rate of production. Recently, we made a first experimental study of overall regulation of storage oil accumulation in oilseed rape, which we analysed by a mathematical method called flux control analysis. This showed that it is the final assembly that is the most important limitation on the biosynthetic process. The assembly process requires several enzyme steps and we have already highlighted one of these, diacylglycerol acyltransferase (DGAT), as being a significant controlling factor. We now wish to examine enzymes, other than DGAT, involved in storage lipid assembly and in supply of component parts. This will enable us to quantify the limitations imposed by different enzymes of the pathway and, furthermore, will provide information to underpin logical steps in genetic manipulation leading to plants with increased oil synthesis and storage capabilities. We will use rape plants where the activity of individual enzymes in the biosynthetic pathway have been changed and quantify the effects on overall oil accumulation. To begin with we will use existing transgenic oilseed rape, with increased enzyme levels, where increases in oil yields have been noted; these are available from our collaborators (Canada, Germany). For enzymes where there are no current transgenic plants available, we will make these and carry out similar analyses. Although our primary focus is on enzymes that increase oil yields, we will also examine the contribution the enzyme phospholipid: diacylglycerol acyltransferase (PDAT) makes to lipid production because this enzyme controls the accumulation of unsaturated oil, which has important dietary implications. In the analogous model plant Arabidopsis, PDAT and DGAT are both important during oil production. Once we have assembled data from these transgenic plants we will have a much better idea of the control of lipid production in oilseed rape. Our quantitative measurements will provide specific targets for future crop improvements. In addition, because we will be monitoring oil yields as well as flux control we will be able to correlate these two measures. Moreover, plants manipulated with multiple genes (gene stacking) will reveal if there are synergistic effects of such strategies. Because no one has yet defined quantitatively the oil synthesis pathway in crops, data produced in the project will have a fundamental impact in basic science. By combining the expertise of three important U.K. labs. with our world-leading international collaborators, this cross-disciplinary project will ensure a significant advance in knowledge of direct application to agriculture.

Carbohydrates, or sugars, are ubiquitous throughout nature and perform a number of important functions in our cells. Carbohydrates can exist in long chains, called polysaccharides, which is how energy is stored from the food we eat, why wood is strong and is responsible for the molecular glue that sticks our cells together. At the other end of the scale, single or a few sugars can be appended to other biomolecules such as proteins and lipids and are important in cell processes such as signalling and defence against pathogens. The structure and sequence of carbohydrates is complex and highly variable, but unlike DNA there is no genetic code that can be read to determine how it should exist. Instead, carbohydrate structure and sequence is defined only the enzymes, nature's catalysts, that make and break-down the carbohydrate molecules. We are interested in an enzyme, called HexD, which cleaves a sugar called N-acetyl galactosamine from substrates. Little work has been done to characterise human HexD, and the substrate on which it acts in cells, and its function, are unknown. However, it has been shown that HexD is found in the synovial fluid of patients suffering from rheumatoid arthritis, and thus understanding HexD at the molecular level could have an important impact on the health of patients suffering from the disease in the longer term. Our preliminary work on HexD suggests it may act on proteins in cells, but further investigations are needed to understand this fully. We have also revealed that HexD has some unprecedented activities, which we will dissect. The over-arching aim of the project is to understand the biological role played by HexD, and we will do this by gaining fundamental insights into how HexD works at the molecular level. We will make HexD in the laboratory and study how it works, test various substrates in order to understand its catalytic activities, and identify proteins with which it interacts in cells. We will also develop specific inhibitors against HexD, which will significantly slow its activity. These inhibitors will be administered to cells, and we will examine the effect on how the cells grow and work, to aid our understanding of the role played by HexD. In addition, we will change (increase and decrease) the levels of HexD in cells and similarly monitor the effect. Overall, these experiments will advance our understanding of the biological function of HexD.

Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.

Controversy surrounds the actual impacts of Atlantic salmon farming on wild salmonid stocks, fed by the lack of direct evidence for or against many potential impacts, with uncertainty an increasing impediment to sustainable industry development and effective management of wild stocks. This applies to the potential impact of the introgression of farm genomes into locally adapted wild populations from breeding of farm escapes. Escapes do occur and are recognized as inevitable, but are a very small fraction of farm stocks and vary in numbers both locally and temporally. The majority of escapees are expected to die without breeding but some do remain in or ascend rivers and spawn. However, a detailed understanding of actual levels of interbreeding and introgression in most rivers is lacking which, along with an understanding of the adaptive differentiation of farm and wild salmon, is required to establish the actual impact of this potential interaction on the productivity and viability of wild populations. Detection and quantification of interbreeding and introgression requires diagnostic markers for farm and wild genomes. Genetic differentiation of farm and wild genomes can evolve through founder effects, selective breeding and domestication selection and is observed in respect of a variety of molecular markers. However, existing molecular markers are not fully diagnostic and regionally constrained in their usefulness. Unfortunately, marker panels screened for useful variation have been small and arbitrary such that they are unlikely to include the most informative loci and to be context specific, limiting their power and transferability. To properly address the issue of introgression molecular markers are required that are highly diagnostic across all farm and wild populations. These markers will be in genomic regions involved in domestication and controlling the expression of selected economic traits. What is known of the genomic architecture of domestication and most economic traits indicates their control is polygenic, making the targeting of specific gene regions in the search for markers difficult. In contrast, recent advances in genomics make possible genome scanning and genome-wide association studies (GWAS) which can provide a high resolution assessment of molecular differentiation between different individuals or populations across the genome. Different GWAS strategies can be employed but two are deemed optimal in the current context. Firstly, a GWAS will be carried out using a new Atlantic salmon SNP (single nucleotide polymorphism) containing 930k nuclear SNPs, recently developed in collaboration with the salmon farming industry. This will be carried out on a broad base of representative farm and wild stocks. Secondly, GWAS will be carried out to identify temporally stable epigenetic DNA-methylation base changes induced by rearing fish in culture by comparing groups of single source wild fish reared in the wild and in culture. The study will deliver the first general understanding of domestication related molecular genetic differentiation between farmed and wild salmon and identify the best markers for identifying farm salmon in the wild and assessing genetic introgression of farm genes into wild populations. The work will deliver a more robust and generally applicable tool for determining the actual levels of escapes and introgression in wild salmon populations. Following field calibration and independent validation, the diagnostic methodology defined in the study is expected to provide the basis for generating the evidence needed to clarify the debate on levels of escapes and introgression and the long term impacts of introgression on population viability. This will help to define more clearly the path forward for the sustainable development of the salmon farming industry in the UK and elsewhere in the North Atlantic region and help to inform management priorities for wild Atlantic salmon stocks.

Life expectancies in the UK have increased rapidly over the last century. If this continues, recent studies have predicted that the majority of babies born since 2000 will live to 100. Our ageing population poses serious economic and medical problems, unless we can find ways of alleviating the process of physiological deterioration and many diseases that are associated with old age. Simple biological measures (or 'biomarkers') capable of illuminating the wider process of ageing and predicting the onset of common diseases of old age could provide important new understanding of the underlying causes of individual variation in ageing rates, as well as interventions to promote healthy ageing. Telomere length (TL) is an exciting candidate biomarker of ageing. Telomeres cap and protect our chromosomes and become shorter with each cell division. When telomeres become very short, cells stop functioning properly, with potentially negative consequences for wider bodily function. Accordingly, the process of telomere attrition is thought to play an important role in the way we age. In humans, telomeres are usually measured in white blood cells, because blood is relatively easy to obtain, and average TL of these cells declines with age. Excitingly, short TL in adulthood predicts various age-related diseases and reduced subsequent survival. However, age only explains a small part of the massive variation in TL among individuals, and we currently do not know why adult TL varies so much. Is it because of genetic or environmental effects on TL at birth, or is it down to differences in growth rates or experiences through early life and adulthood which affect the rate of telomere shortening? To answer this question we need blood samples and information over the entire lifetimes of individuals. This has not been possible in humans because we are so long lived. Furthermore, there are considerable differences in the telomere biology of short-lived and long-lived mammals, so laboratory mice may be poor models for humans. In this project, we will use a remarkably detailed long-term study of Soay sheep on St Kilda to tackle the question of how and why TL varies across the entire lifespan and what this means for the ageing process. It might seem odd to be using wild sheep on a remote island for such a purpose. In fact, the telomere biology of sheep and humans is similar, and the Soay sheep are one of the most closely monitored populations of mammals anywhere in the world. Since 1985, every sheep has been individually marked and followed closely across its lifetime, so we know how quickly they grew, when they bred, where they lived, when they died and we have detailed information on their genetics and the environmental conditions they experience. Importantly, we also regularly re-capture these animals and have collected blood samples repeatedly from around 3000 individuals all the way from birth to death. We will measure TL from archived blood samples to test whether differences in TL in late adulthood are mainly the result of differences in TL at birth or in telomere loss thereafter. We will also test how genes and environment during development influence TL and how natural selection acts on variation in TL. The uniquely detailed, life-long nature of our study will provide the first tests of the causes of individual variation in telomere attrition rates across the entire lifespan of a long-lived mammal. We will also extend the fieldwork on St Kilda to collect samples for more extensive telomere and immunological analyses. Laboratory studies show that a few very short telomeres are enough to compromise cell function, and in white blood cells this could compromise our immune system. Using newly-collected field data and blood samples, we will test both of these predictions outside of the lab for the first time, shedding new light on how changes in TL may influence our ability to fight disease, maintain health and survive in later adulthood.

All animals need to make the most of new opportunities or deal with changing environmental conditions. These changes may be short-term such as seasonal change, or long-term shifts such as climate change, and often impact the availability of food resources and, potentially, survival. Broadly, two different strategies might be used to increase access to resources in a changing environment. Animals might develop new solutions to problems (innovation), and thus find new resources themselves, or they might observe others and copy successful solutions. The latter, called social learning, is expected to be much more frequent than innovation, allows new behaviours to spread rapidly between individuals and is thought to be fundamental in forming traditions. Social learning has long fascinated biologists and anthropologists; understanding how behaviours spread and traditions are maintained in animals can shed light on the factors promoting complex culture in humans. An important determinant of social learning is the social organisation of the population in which learning occurs. It was long thought that only humans could exhibit highly developed cultural transmission due to their capacity for communication and learning that is facilitated by long-term social bonds. However recent research has found locally maintained cultural behaviour in a wide range of animals. Further, social network analysis in both human and other animal populations has allowed population structure to be accurately measured. Thus, using social networks to map the spread of new behaviours provides an exciting opportunity to understand this important learning process. In this study, we will study the spread of novel information in wild populations of a common bird, the great tit. All individuals in our large study population are tagged with microchips allowing us to track them automatically; our pilot data shows that they will learn socially. We will develop devices where one of two simple solutions provides access to food, and train an individual to solve one solution on the task in captivity before releasing it back into the woodland where we will place a number of these devices. Using this approach, we will be able to track who has learnt, from whom they learnt, and which of the two solutions they learnt. Using the social network of this population, we will track the spread of the new behaviours, and determine what characteristics made some individuals more important in spreading them. By training different individuals on the two different solutions, we will also see how local traditions develop and are maintained. Not all traditions or behaviours are advantageous. For example, in humans it has been shown that obesity can spread through friendship groups. In the second phase of this project we will alter the reward to different solutions of the task by replacing the popular solution with a low reward (peanut granules instead of a worm), maintaining the high reward on the less popular solution. This will test whether bad traditions are maintained through social reinforcement where individuals blindly copy the majority even when better solutions exist. Finally, we will develop some new technology that will predict what solution new individuals should be learning based upon the behavior of the group they belong to. By changing the behaviour at the device in response, we will then test in detail what elements of the behaviour observed in others is used when social learning. This will be the first time that anyone has used an active device to directly manipulate the behaviour of wild animals in this way. This will itself advance scientists' abilities to understand what rules individuals follow when making decisions such as who to copy and when. Such knowledge will be widely applicable across disciplines, for example in providing new opportunities for active conservation of threatened species by introducing behaviours that improve survival.

Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.

For the UK to achieve net carbon neutrality by 2050, it is estimated that the mix of Greenhouse Gas Removal (GGR) technologies required will equate to ca. 35 M tonnes of carbon (MtC) p.a. Biochar can potentially make a major contribution both to this target and the adoption of farming practices described by the Committee on Climate Change (2020) to achieve a 64% reduction by 2050 in greenhouse gas emissions across agriculture, land use and peatlands by 64% from the 2017 level of 16 MtC. However, there are some significant challenges to overcome. There is limited availability of virgin wood to produce biochar and there are no large-scale production plants operating in the UK. Further, as well as economic viability and societal acceptability, there are concerns over biochar stability with initial degradation occurring over relatively short timescales. We propose to conduct the most ambitious and comprehensive demonstration programme to date involving arable and grassland, woodland, contaminated land, and where soil erosion control is required. Using over 200 tonnes of biochar, we will address uncertainties regarding the extent and scope of deployment and its stability with respect to carbon sequestration, together with quantifying effects on ecosystem services. The proposed research programme is highly inter-disciplinary, bridging engineering, geoscience, bioscience, social science and techno-economics, specifically designed to provide answers to the key challenges outlined and establish whether biochar can make a significant contribution to meet the UK's 2050 GGR target . The quantitative approach that we will adopt based on internationally leading science represents a step-change for biochar research in the UK, which has focussed primarily on agricultural benefits and not addressed the key challenges regarding carbon sequestration that are needed to reduce the uncertainty for policy development. Alternative bio-derived feedstocks that will significantly increase the production potential by >1 MtC p.a, will be identified. Two of our industrial partners, CEG and CPL operate demonstration and commercial plants, making them ideally placed to establish biochar production at scale in the UK. The extensive trials will provide a sound basis for establishing the potential for biochar deployment across agriculture, contaminated and reclaimed land and woodland, enabling regional and national scale effects to be quantified. To date, most field trials have been relatively localised and short-term. We aim to deploy char in large-scale farming and land management scenarios where the effects of 'real-world' management practices on the behaviour of char will be evaluated. Our excellent links with the farming sector, including the Agriculture and Horticulture Development Board and the National Farmers' Union, will provide the springboard to explore a wide range of stakeholder perspectives on biochar's role in GGR to aid policy development. . The Demonstrator will address concerns over environmental health and soil ecosystem service functioning and will provide the first comprehensive assessment of biochar stability in the UK and its impact on greenhouse gas soil emissions, with our international leading biological science and analytical capabilities. This will enable robust policy to be developed in which payments are based on the amount of carbon sequestered over extended timescales. Our business models will be based on our integrated life cycle and techno-economic analysis, identifying the carbon prices required to make deployment feasible and incorporating co-benefits of biochar use in agriculture. The Demonstrator will provide the Hub with all the necessary scientific, technological, environmental, economic and societal evidence to enable biochar deployment to be assessed in relation to other GGR approaches.