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  • Open Access
    Authors: 
    Trifunović, Srećko R.; Marković, Zorica; Sladić, Dušan; Anđelković, Katarina K.; Sabo, Tibor; Minić, Dragica M.;
    Publisher: Serbian Chemical Soc, Belgrade
    Country: Serbia

    Square planar complexes of Ni(II) and Cu(II) with potassium 3-dithiocarboxy-3-aza-5-aminopentanoate have been prepared by direct synthesis. The obtained neutral complexes were characterized by elemental analysis, magnetic susceptibility measurements, infrared and electronic spectra. The thermal behaviour of both the Ni(II) and Cu(II) complexes, and the ligand itself was investigated by DSC and TG. Direktnom sintezom između nikal(II)- i bakar(II)- soli i kalijum 3-ditiokarboksi-3-aza-5-aminopentanoata nagrađeni su odgovarajući kompleksi kvadratno-planarne strukture. Izolovani neutralni kompleksi karakterisani su elementalnom analizom, infracrvenom i elektronskom apsorpcionom spektroskopijom, kao i merenjem magnetnih susceptibiliteta. Termičko ponašanje kompleksa i liganda ispitivano je primenom DSC i TG metoda.

  • Open Access
    Authors: 
    Una Glamočlija; Subhash Padhye; Selma Špirtović-Halilović; Amar Osmanović; Elma Veljović; Sunčica Roca; Irena Novaković; Boris Mandić; Iztok Turel; Jakob Kljun; +6 more
    Publisher: MDPI
    Countries: Serbia, Croatia, Slovenia, Croatia

    Thymoquinone (TQ), a natural compound with antimicrobial and antitumor activity, was used as the starting molecule for the preparation of 3-aminothymoquinone (ATQ) from which ten novel benzoxazole derivatives were prepared and characterized by elemental analysis, IR spectroscopy, mass spectrometry and NMR (1H, 13C) spectroscopy in solution. The crystal structure of 4-methyl-2-phenyl-7-isopropyl-1,3-benzoxazole-5-ol (1a) has been determined by X-ray diffraction. All compounds were tested for their antibacterial, antifungal and antitumor activities. TQ and ATQ showed better antibacterial activity against tested Gram-positive and Gram-negative bacterial strains than benzoxazoles. ATQ had the most potent antifungal effect against Candida albicans, Saccharomyces cerevisiae and Aspergillus brasiliensis. Three benzoxazole derivatives and ATQ showed the highest antitumor activities. The most potent was 2-(4-fluorophenyl)-4-methyl-7-isopropyl-1,3-benzoxazole-5-ol (1f). Western blot analyses have shown that this compound inhibited phosphorylation of protein kinase B (Akt) and Insulin-like Growth Factor-1 Receptor (IGF1R &beta ) in HeLa and HepG2 cells. The least toxic compound against normal fibroblast cells, which maintains similar antitumor activities as TQ, was 2-(4-chlorophenyl)-4-methyl-7-isopropyl-1,3-benzoxazole-5-ol (1e). Docking studies indicated that 1e and 1f have significant effects against selected receptors playing important roles in tumour survival.

  • Open Access
    Authors: 
    Zizic, Jovana B.; Vukovic, Nenad L.; Jadranin, Milka; Anđelković, Boban D.; Tešević, Vele; Kacaniova, Miroslava M.; Sukdolak, Slobodan B.; Marković, Snežana D.;
    Publisher: Wiley, Hoboken
    Country: Serbia
    Project: MESTD | Preclinical investigation... (41010), MESTD | Natural products of wild,... (172053)

    BACKGROUND Propolis is a complex resinous sticky substance that honeybees collect from buds and exudates of various plants. Owing to its versatile biological and pharmacological activities, propolis is widely used in medicines, cosmetics and foods. The aim of this study was to evaluate the cytotoxic and antioxidative effects of various ethanolic extracts of propolis (EEPs) on human colon cancer cell line HCT-116 and compare them with their composition determined by HPLC-DAD. RESULTS The most abundant flavonoids in all samples were chrysin, pinocembrin and galangin (12.697-40.811 mu gmg(-1)), while the main phenolic acids were caffeic acid, ferulic acid and isoferulic acid. Dose- and time-dependent inhibition of growth of HCT-116 cells was observed for all propolis samples, with IC50 values ranging from 26.33 to 143.09 mu gmL(-1). Differences in cytotoxic activity of propolis samples were associated with differences in their composition. All EEP samples reduced both superoxide anion radical and nitrite levels and also had strong DPPH-scavenging activity. CONCLUSION All tested propolis samples had pronounced cytotoxic and antioxidative activities. This is the peer-reviewed version of the following article: Žižić, J. B.; Vuković, N. L.; Jadranin, M.; Anđelković, B. D.; Tešević, V.; Kacaniova, M. M.; Sukdolak, S. B.; Markovic, S. D. Chemical Composition, Cytotoxic and Antioxidative Activities of Ethanolic Extracts of Propolis on HCT-116 Cell Line. Journal of the Science of Food and Agriculture 2013, 93 (12), 3001–3009. [https://doi.org/10.1002/jsfa.6132] [http://cer.ihtm.bg.ac.rs/handle/123456789/1213]

  • Open Access
    Authors: 
    Guzelmeric, Etil; Ristivojević, Petar; Trifković, Jelena; Dastan, Tugce; Yilmaz, Ozlem; Cengiz, Ozlem; Yesilada, Erdem;
    Publisher: Elsevier Science Bv, Amsterdam
    Country: Serbia
    Project: MESTD | Structure-properties rela... (172017)

    Supplementary data for: [https://doi.org/10.1016/j.lwt.2017.08.060] Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2552] Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/2989]

  • Open Access
    Authors: 
    Katarina Smiljanic; Danijela Apostolovic; Snežana Trifunović; Jana Ognjenovic; Marija Perusko; Luka Mihajlovic; Lidija Burazer; M. van Hage; T. Cirkovic Velickovic;
    Publisher: Blackwell Publishing Ltd
    Countries: Belgium, Serbia, Serbia
    Project: MESTD | Molecular properties and ... (172024), EC | FCUB-ERA (256716)

    Background Short ragweed (Ambrosia artemisiifolia) allergies affect more than 36 million people annually. Ragweed pollen grains release subpollen particles (SPP) of respirable size upon hydration or a change in air electrical conditions. The aim of this study was to characterize the proteomes and allergomes of short ragweed SPP and total pollen protein extract (TOT), and compare their effects with those of standard aqueous pollen protein extract (APE) using sera from short ragweed pollen-sensitized patients. Methods Quantitative 2D gel-based and shotgun proteomics, 1D and 2D immunoblotting, and quantitative ELISA were applied. Novel SPP extraction and preparation protocols enabled appropriate sample preparation and further downstream analysis by quantitative proteomics. Results The SPP fraction contained the highest proportion (94%) of the allergome, with the largest quantities of the minor Amb a 4 and major Amb a 1 allergens, and as unique, NADH dehydrogenases. APE was the richest in Amb a 6, Amb a 5 and Amb a 3, and TOT fraction was the richest in the Amb a 8 allergens (89% and 83% of allergome, respectively). Allergenic potency correlated well among the three fractions tested, with 1D immunoblots demonstrating a slight predominance of IgE reactivity to SPP compared to TOT and APE. However, the strongest IgE binding in ELISA was noted against APE. New allergenic candidates, phosphoglycerate mutase and phosphoglucomutase, were identified in all the three pollen fractions. Enolase, UTP-glucose-1-phosphate uridylyltransferase and polygalacturonase were observed in SPP and TOT fractions as novel allergens of the short ragweed pollen, as previously described. Conclusion and Clinical Relevance We demonstrated that the complete major (Amb a 1 and 11) and almost all minor (Amb a 3, 4, 5, 6, 8 and 9) short ragweed pollen allergen repertoire as well as NADH oxidases are present in SPP, highlighting an important role for SPP in allergic sensitization to short ragweed. This is the peer-reviewed version of the following article: Smiljanic, K.; Apostolovic, D.; Trifunovic, S.; Ognjenovic, J.; Perusko, M.; Mihajlovic, L.; Burazer, L.; van Hage, M.; Cirkovic Velickovic, T. Subpollen Particles Are Rich Carriers of Major Short Ragweed Allergens and NADH Dehydrogenases: Quantitative Proteomic and Allergomic Study. Clinical and Experimental Allergy 2017, 47 (6), 815–828. [https://doi.org/10.1111/cea.12874] Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/3127]

  • Open Access
    Authors: 
    Jana Ognjenovic; Omar Ziyad Tantoush; Ratko M. Jankov; Ćirković Tanja Veličković; Jelena Vukmirica;
    Country: Serbia
    Project: MESTD | Molecular properties and ... (172024), EC | FCUB-ERA (256716)

    The conditions required for the isolation of high quality total RNA from European linden (Tilia cordata) leaves and pollen were determined. Pure total RNA was isolated from linden leaves utilizing a Qiagen plant mini kit, while the total RNA isolated from linden pollen using this method was degraded. Successful isolation of total RNA from both linden pollen and leaves, however, was achieved following TRIzol (TM) preparation of the total RNA. The total RNA isolated using TRIzol (TM) was contaminated with genomic DNA but treatment with the enzyme DNase, in solution or on-column, efficiently removed the genomic DNA. Furthermore, the conditions for the elimination of genomic DNA contamination on-column and isolation of pure total RNA from leaves were optimized. The isolated total RNA from both leaves and pollen was used successfully in first-and second-strand cDNA synthesis reactions and in a reverse transcription polymerase chain reaction (RT-PCR), demonstrating that the total RNA isolated using this method was functional. In conclusion, pure and functional total RNA from T. cordata leaves and pollen (27.8 +/- 7.9 mu g g(-1) leaves; 25.7 +/- 1.1 mu g g(-1) pollen) could be obtained and was suitable for application in further molecular biology studies. Uspostavljeni su uslovi za izolovanje ukupne RNK iz lišća i polena evropske lipe (Tilia cordata). Korišćenjem komercijalno dostupnog pribora za izolovanje RNK iz biljaka izolovana je čista ukupna RNK iz lišća lipe, dok je korišćenjem iste metode dobijena degradirana RNK iz polena lipe. Uspešno izolovanje RNK iz lišća i polena je dobijeno korišćenjem TRIzol reagensa. RNK izolovana ovim metodom je kontaminirana genomskom DNK, koja je uspešno eliminisana korišćenjem enzima DNaze. Dalje su optimizovani i uslovi uklanjanja genomske DNK pomoću DNaze. Izolovana ukupna RNK iz oba izvora je dalje uspešno iskorišćena za sintezu prvog i drugog lanca klonske DNK, kao i u reverzno-transkriptivnoj PCR reakciji, dokazujući time da je korišćenjem ovog metoda izolovana funkcionalna ukupna RNK. U zaključku, dobijena je čista i funkcionalna RNK iz lišća i polena T. cordata (27,8±7,9 μg g-1 lišća; 25,7±1,1 μg g-1 polena) koja se može koristiti u daljim molekularno-biološkim istraživanjima.

  • Restricted
    Authors: 
    Boris Pejin; Armandodoriano Bianco; Steven G. Newmaster; Marko Sabovljevic; Lj-J. Vujisic; Vele Tešević; V. Vajs; S. De Rosa;
    Publisher: Taylor & Francis Ltd, Abingdon
    Countries: Italy, Serbia

    The chemical composition of Rhodobryum ontariense (Kindb.) Kindb. has not been previously investigated. Fatty acids of this moss were analysed qualitatively and quantitatively with an aim to identify its corresponding pattern. A total of eight fatty acids were identified including two acetylenic ones: 9,12,15-octadecatrien-6-ynoic acid (42.26%), alpha-linolenic acid (20.32%), palmitic acid (14.31%), 9,12-octadecadienoic-6-ynoic acid (13.31%), linoleic acid (5.25%), oleic acid (2.47%), stearic acid (1.14%) and gamma-linolenic acid (0.92%). To our knowledge, this is the first record of acetylenic fatty acids in the genus Rhodobryum. In general, acetylenic fatty acids vary considerably among different moss groups and have been used as a chemotaxonomic character in bryophyte classifications. Other species of Rhodobryum from Asia have been traditionally used in ethno medicine by indigenous cultures. Two fatty acids of those reported here, 9,12,15-octadecatrien-6-ynoic and alpha-linolenic acid, have known cardio protective activity, which supports respective claims of traditional herbal use of these mosses.

  • Open Access
    Authors: 
    Milena Trmčić; Frances L. Chadbourne; Paul Brear; Paul W. Denny; Steven L. Cobb; David R. W. Hodgson;
    Publisher: Royal Soc Chemistry, Cambridge
    Countries: United Kingdom, Serbia

    We recently reported the use of PSCl3 for the thiophosphorylation of alkylamines where the resulting N-thiophosphoramidate ions could be readily S-alkylated (Chem. Commun., 2011, 47, 6156-6158.). Herein we report the development of this methodology using amino acid, amino sugar, aminonucleoside and aniline substrates. The hydrolysis properties of N-thiophosphoramidate ions and their reactivities towards alkylating agents are also explored. In addition, we demonstrate the application of our approach to the preparation of a small library of compounds, including quinoline-based N,S-dialkylthiophosphoramidates which were tested for antileishmanial activity. This is the peer-reviewed version of the following article: Trmcic, M. and Chadbourne, F.L. and Brear, P.M. and Denny, P.W. and Cobb, S.L. and Hodgson, D.R.W., 2013. Aqueous synthesis of N,S,-dialkylthiophosphoramidates: design, optimisation and application to library construction and antileishmanial testing. Organic and biomolecular chemistry. 11, 2660-2675. [http://doi.org/10.1039/c3ob27448a] Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/3468]

  • Open Access
    Authors: 
    Sabo, Tibor; Trifunović, Srećko R.;
    Publisher: Serbian Chemical Soc, Belgrade
    Country: Serbia

    The meridional geometrical isomer of uns-cis-(ethylenediamine-N-N'-di-3-propionato)(S-norleucinato)cobalt(III) complex has been prepared by the reaction of sodium uns-cis-(ethylenediamine-N-N'-di-3-propionato)(carbonato)cobaltate(III) with S-norleucine at 75 degrees C. The complex was isolated choromatographically and characterized by elemental analyses, electron absorption and infrared spectroscopy. Sintetisan je i okarakterisan meridijalni izomer uns-cis-(etilendiamin-N-N'-di-3-propionato)(S-norleucinato)kobalt(III) hemihidrat. Kompleks je dobiven reakcijom natrijum-uns-cis-(etilendiamin-N-N'-di-3-propionato)kobaltata(III) dihidratai S-norleucina na 75 ºC. Kompleks je izolovan hromatografski i okarakterisan elementalnom analizom, elektronsko-apsorpcionom i infracrvenom spektroskopijom.

  • Open Access
    Authors: 
    Goran N. Kaludjerović; Frank W. Heinemann; Vukadin M. Leovac; Srećko R. Trifunović; Tibor J. Sabo;
    Publisher: International Union of Crystallography (IUCr)
    Country: Serbia

    In the anion of the title compound, (C2H10N2)[VO(H2O)(C3H2O4)(2)] or H(2)en[VO(mal)(2)H2O], vanadium(IV) is distorted-octahedrally coordinated by six donor O atoms. The two malonate ligands are situated in the equatorial plane, whereas the oxo and the water ligand occupy axial positions. The apical V=O bond exhibits a strong trans influence. The anion possesses crystallographically imposed C-2 symmetry, with the central V atom, the oxo and the water ligand lying on the twofold axis.

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19 Research products, page 1 of 2
  • Open Access
    Authors: 
    Trifunović, Srećko R.; Marković, Zorica; Sladić, Dušan; Anđelković, Katarina K.; Sabo, Tibor; Minić, Dragica M.;
    Publisher: Serbian Chemical Soc, Belgrade
    Country: Serbia

    Square planar complexes of Ni(II) and Cu(II) with potassium 3-dithiocarboxy-3-aza-5-aminopentanoate have been prepared by direct synthesis. The obtained neutral complexes were characterized by elemental analysis, magnetic susceptibility measurements, infrared and electronic spectra. The thermal behaviour of both the Ni(II) and Cu(II) complexes, and the ligand itself was investigated by DSC and TG. Direktnom sintezom između nikal(II)- i bakar(II)- soli i kalijum 3-ditiokarboksi-3-aza-5-aminopentanoata nagrađeni su odgovarajući kompleksi kvadratno-planarne strukture. Izolovani neutralni kompleksi karakterisani su elementalnom analizom, infracrvenom i elektronskom apsorpcionom spektroskopijom, kao i merenjem magnetnih susceptibiliteta. Termičko ponašanje kompleksa i liganda ispitivano je primenom DSC i TG metoda.

  • Open Access
    Authors: 
    Una Glamočlija; Subhash Padhye; Selma Špirtović-Halilović; Amar Osmanović; Elma Veljović; Sunčica Roca; Irena Novaković; Boris Mandić; Iztok Turel; Jakob Kljun; +6 more
    Publisher: MDPI
    Countries: Serbia, Croatia, Slovenia, Croatia

    Thymoquinone (TQ), a natural compound with antimicrobial and antitumor activity, was used as the starting molecule for the preparation of 3-aminothymoquinone (ATQ) from which ten novel benzoxazole derivatives were prepared and characterized by elemental analysis, IR spectroscopy, mass spectrometry and NMR (1H, 13C) spectroscopy in solution. The crystal structure of 4-methyl-2-phenyl-7-isopropyl-1,3-benzoxazole-5-ol (1a) has been determined by X-ray diffraction. All compounds were tested for their antibacterial, antifungal and antitumor activities. TQ and ATQ showed better antibacterial activity against tested Gram-positive and Gram-negative bacterial strains than benzoxazoles. ATQ had the most potent antifungal effect against Candida albicans, Saccharomyces cerevisiae and Aspergillus brasiliensis. Three benzoxazole derivatives and ATQ showed the highest antitumor activities. The most potent was 2-(4-fluorophenyl)-4-methyl-7-isopropyl-1,3-benzoxazole-5-ol (1f). Western blot analyses have shown that this compound inhibited phosphorylation of protein kinase B (Akt) and Insulin-like Growth Factor-1 Receptor (IGF1R &beta ) in HeLa and HepG2 cells. The least toxic compound against normal fibroblast cells, which maintains similar antitumor activities as TQ, was 2-(4-chlorophenyl)-4-methyl-7-isopropyl-1,3-benzoxazole-5-ol (1e). Docking studies indicated that 1e and 1f have significant effects against selected receptors playing important roles in tumour survival.

  • Open Access
    Authors: 
    Zizic, Jovana B.; Vukovic, Nenad L.; Jadranin, Milka; Anđelković, Boban D.; Tešević, Vele; Kacaniova, Miroslava M.; Sukdolak, Slobodan B.; Marković, Snežana D.;
    Publisher: Wiley, Hoboken
    Country: Serbia
    Project: MESTD | Preclinical investigation... (41010), MESTD | Natural products of wild,... (172053)

    BACKGROUND Propolis is a complex resinous sticky substance that honeybees collect from buds and exudates of various plants. Owing to its versatile biological and pharmacological activities, propolis is widely used in medicines, cosmetics and foods. The aim of this study was to evaluate the cytotoxic and antioxidative effects of various ethanolic extracts of propolis (EEPs) on human colon cancer cell line HCT-116 and compare them with their composition determined by HPLC-DAD. RESULTS The most abundant flavonoids in all samples were chrysin, pinocembrin and galangin (12.697-40.811 mu gmg(-1)), while the main phenolic acids were caffeic acid, ferulic acid and isoferulic acid. Dose- and time-dependent inhibition of growth of HCT-116 cells was observed for all propolis samples, with IC50 values ranging from 26.33 to 143.09 mu gmL(-1). Differences in cytotoxic activity of propolis samples were associated with differences in their composition. All EEP samples reduced both superoxide anion radical and nitrite levels and also had strong DPPH-scavenging activity. CONCLUSION All tested propolis samples had pronounced cytotoxic and antioxidative activities. This is the peer-reviewed version of the following article: Žižić, J. B.; Vuković, N. L.; Jadranin, M.; Anđelković, B. D.; Tešević, V.; Kacaniova, M. M.; Sukdolak, S. B.; Markovic, S. D. Chemical Composition, Cytotoxic and Antioxidative Activities of Ethanolic Extracts of Propolis on HCT-116 Cell Line. Journal of the Science of Food and Agriculture 2013, 93 (12), 3001–3009. [https://doi.org/10.1002/jsfa.6132] [http://cer.ihtm.bg.ac.rs/handle/123456789/1213]

  • Open Access
    Authors: 
    Guzelmeric, Etil; Ristivojević, Petar; Trifković, Jelena; Dastan, Tugce; Yilmaz, Ozlem; Cengiz, Ozlem; Yesilada, Erdem;
    Publisher: Elsevier Science Bv, Amsterdam
    Country: Serbia
    Project: MESTD | Structure-properties rela... (172017)

    Supplementary data for: [https://doi.org/10.1016/j.lwt.2017.08.060] Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2552] Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/2989]

  • Open Access
    Authors: 
    Katarina Smiljanic; Danijela Apostolovic; Snežana Trifunović; Jana Ognjenovic; Marija Perusko; Luka Mihajlovic; Lidija Burazer; M. van Hage; T. Cirkovic Velickovic;
    Publisher: Blackwell Publishing Ltd
    Countries: Belgium, Serbia, Serbia
    Project: MESTD | Molecular properties and ... (172024), EC | FCUB-ERA (256716)

    Background Short ragweed (Ambrosia artemisiifolia) allergies affect more than 36 million people annually. Ragweed pollen grains release subpollen particles (SPP) of respirable size upon hydration or a change in air electrical conditions. The aim of this study was to characterize the proteomes and allergomes of short ragweed SPP and total pollen protein extract (TOT), and compare their effects with those of standard aqueous pollen protein extract (APE) using sera from short ragweed pollen-sensitized patients. Methods Quantitative 2D gel-based and shotgun proteomics, 1D and 2D immunoblotting, and quantitative ELISA were applied. Novel SPP extraction and preparation protocols enabled appropriate sample preparation and further downstream analysis by quantitative proteomics. Results The SPP fraction contained the highest proportion (94%) of the allergome, with the largest quantities of the minor Amb a 4 and major Amb a 1 allergens, and as unique, NADH dehydrogenases. APE was the richest in Amb a 6, Amb a 5 and Amb a 3, and TOT fraction was the richest in the Amb a 8 allergens (89% and 83% of allergome, respectively). Allergenic potency correlated well among the three fractions tested, with 1D immunoblots demonstrating a slight predominance of IgE reactivity to SPP compared to TOT and APE. However, the strongest IgE binding in ELISA was noted against APE. New allergenic candidates, phosphoglycerate mutase and phosphoglucomutase, were identified in all the three pollen fractions. Enolase, UTP-glucose-1-phosphate uridylyltransferase and polygalacturonase were observed in SPP and TOT fractions as novel allergens of the short ragweed pollen, as previously described. Conclusion and Clinical Relevance We demonstrated that the complete major (Amb a 1 and 11) and almost all minor (Amb a 3, 4, 5, 6, 8 and 9) short ragweed pollen allergen repertoire as well as NADH oxidases are present in SPP, highlighting an important role for SPP in allergic sensitization to short ragweed. This is the peer-reviewed version of the following article: Smiljanic, K.; Apostolovic, D.; Trifunovic, S.; Ognjenovic, J.; Perusko, M.; Mihajlovic, L.; Burazer, L.; van Hage, M.; Cirkovic Velickovic, T. Subpollen Particles Are Rich Carriers of Major Short Ragweed Allergens and NADH Dehydrogenases: Quantitative Proteomic and Allergomic Study. Clinical and Experimental Allergy 2017, 47 (6), 815–828. [https://doi.org/10.1111/cea.12874] Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/3127]

  • Open Access
    Authors: 
    Jana Ognjenovic; Omar Ziyad Tantoush; Ratko M. Jankov; Ćirković Tanja Veličković; Jelena Vukmirica;
    Country: Serbia
    Project: MESTD | Molecular properties and ... (172024), EC | FCUB-ERA (256716)

    The conditions required for the isolation of high quality total RNA from European linden (Tilia cordata) leaves and pollen were determined. Pure total RNA was isolated from linden leaves utilizing a Qiagen plant mini kit, while the total RNA isolated from linden pollen using this method was degraded. Successful isolation of total RNA from both linden pollen and leaves, however, was achieved following TRIzol (TM) preparation of the total RNA. The total RNA isolated using TRIzol (TM) was contaminated with genomic DNA but treatment with the enzyme DNase, in solution or on-column, efficiently removed the genomic DNA. Furthermore, the conditions for the elimination of genomic DNA contamination on-column and isolation of pure total RNA from leaves were optimized. The isolated total RNA from both leaves and pollen was used successfully in first-and second-strand cDNA synthesis reactions and in a reverse transcription polymerase chain reaction (RT-PCR), demonstrating that the total RNA isolated using this method was functional. In conclusion, pure and functional total RNA from T. cordata leaves and pollen (27.8 +/- 7.9 mu g g(-1) leaves; 25.7 +/- 1.1 mu g g(-1) pollen) could be obtained and was suitable for application in further molecular biology studies. Uspostavljeni su uslovi za izolovanje ukupne RNK iz lišća i polena evropske lipe (Tilia cordata). Korišćenjem komercijalno dostupnog pribora za izolovanje RNK iz biljaka izolovana je čista ukupna RNK iz lišća lipe, dok je korišćenjem iste metode dobijena degradirana RNK iz polena lipe. Uspešno izolovanje RNK iz lišća i polena je dobijeno korišćenjem TRIzol reagensa. RNK izolovana ovim metodom je kontaminirana genomskom DNK, koja je uspešno eliminisana korišćenjem enzima DNaze. Dalje su optimizovani i uslovi uklanjanja genomske DNK pomoću DNaze. Izolovana ukupna RNK iz oba izvora je dalje uspešno iskorišćena za sintezu prvog i drugog lanca klonske DNK, kao i u reverzno-transkriptivnoj PCR reakciji, dokazujući time da je korišćenjem ovog metoda izolovana funkcionalna ukupna RNK. U zaključku, dobijena je čista i funkcionalna RNK iz lišća i polena T. cordata (27,8±7,9 μg g-1 lišća; 25,7±1,1 μg g-1 polena) koja se može koristiti u daljim molekularno-biološkim istraživanjima.

  • Restricted
    Authors: 
    Boris Pejin; Armandodoriano Bianco; Steven G. Newmaster; Marko Sabovljevic; Lj-J. Vujisic; Vele Tešević; V. Vajs; S. De Rosa;
    Publisher: Taylor & Francis Ltd, Abingdon
    Countries: Italy, Serbia

    The chemical composition of Rhodobryum ontariense (Kindb.) Kindb. has not been previously investigated. Fatty acids of this moss were analysed qualitatively and quantitatively with an aim to identify its corresponding pattern. A total of eight fatty acids were identified including two acetylenic ones: 9,12,15-octadecatrien-6-ynoic acid (42.26%), alpha-linolenic acid (20.32%), palmitic acid (14.31%), 9,12-octadecadienoic-6-ynoic acid (13.31%), linoleic acid (5.25%), oleic acid (2.47%), stearic acid (1.14%) and gamma-linolenic acid (0.92%). To our knowledge, this is the first record of acetylenic fatty acids in the genus Rhodobryum. In general, acetylenic fatty acids vary considerably among different moss groups and have been used as a chemotaxonomic character in bryophyte classifications. Other species of Rhodobryum from Asia have been traditionally used in ethno medicine by indigenous cultures. Two fatty acids of those reported here, 9,12,15-octadecatrien-6-ynoic and alpha-linolenic acid, have known cardio protective activity, which supports respective claims of traditional herbal use of these mosses.

  • Open Access
    Authors: 
    Milena Trmčić; Frances L. Chadbourne; Paul Brear; Paul W. Denny; Steven L. Cobb; David R. W. Hodgson;
    Publisher: Royal Soc Chemistry, Cambridge
    Countries: United Kingdom, Serbia

    We recently reported the use of PSCl3 for the thiophosphorylation of alkylamines where the resulting N-thiophosphoramidate ions could be readily S-alkylated (Chem. Commun., 2011, 47, 6156-6158.). Herein we report the development of this methodology using amino acid, amino sugar, aminonucleoside and aniline substrates. The hydrolysis properties of N-thiophosphoramidate ions and their reactivities towards alkylating agents are also explored. In addition, we demonstrate the application of our approach to the preparation of a small library of compounds, including quinoline-based N,S-dialkylthiophosphoramidates which were tested for antileishmanial activity. This is the peer-reviewed version of the following article: Trmcic, M. and Chadbourne, F.L. and Brear, P.M. and Denny, P.W. and Cobb, S.L. and Hodgson, D.R.W., 2013. Aqueous synthesis of N,S,-dialkylthiophosphoramidates: design, optimisation and application to library construction and antileishmanial testing. Organic and biomolecular chemistry. 11, 2660-2675. [http://doi.org/10.1039/c3ob27448a] Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/3468]

  • Open Access
    Authors: 
    Sabo, Tibor; Trifunović, Srećko R.;
    Publisher: Serbian Chemical Soc, Belgrade
    Country: Serbia

    The meridional geometrical isomer of uns-cis-(ethylenediamine-N-N'-di-3-propionato)(S-norleucinato)cobalt(III) complex has been prepared by the reaction of sodium uns-cis-(ethylenediamine-N-N'-di-3-propionato)(carbonato)cobaltate(III) with S-norleucine at 75 degrees C. The complex was isolated choromatographically and characterized by elemental analyses, electron absorption and infrared spectroscopy. Sintetisan je i okarakterisan meridijalni izomer uns-cis-(etilendiamin-N-N'-di-3-propionato)(S-norleucinato)kobalt(III) hemihidrat. Kompleks je dobiven reakcijom natrijum-uns-cis-(etilendiamin-N-N'-di-3-propionato)kobaltata(III) dihidratai S-norleucina na 75 ºC. Kompleks je izolovan hromatografski i okarakterisan elementalnom analizom, elektronsko-apsorpcionom i infracrvenom spektroskopijom.

  • Open Access
    Authors: 
    Goran N. Kaludjerović; Frank W. Heinemann; Vukadin M. Leovac; Srećko R. Trifunović; Tibor J. Sabo;
    Publisher: International Union of Crystallography (IUCr)
    Country: Serbia

    In the anion of the title compound, (C2H10N2)[VO(H2O)(C3H2O4)(2)] or H(2)en[VO(mal)(2)H2O], vanadium(IV) is distorted-octahedrally coordinated by six donor O atoms. The two malonate ligands are situated in the equatorial plane, whereas the oxo and the water ligand occupy axial positions. The apical V=O bond exhibits a strong trans influence. The anion possesses crystallographically imposed C-2 symmetry, with the central V atom, the oxo and the water ligand lying on the twofold axis.