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Additional file 1. Appendix

Additional file 13. Data.

Additional file 3: Table S3. GR methylation in U and C regions for patients by outcome.

Additional file 23. Microarray image data from replicate 3 of MCF-7 breast cancer cells grown in static culture.

Additional file 12: Table S1. Total number of ones, zeros and nulls in the sparse matrix for the 163 TFs used in this study.

An excel file containing the relative abundance of rumen bacterial community and differentially abundant microbial functions. (XLSX 27 kb)

Additional file 5: Table S5. Proteins differentially expressed between non-responders (NR) and responders (PR+TR).

Additional file 2. Data of individual patients.

We document the fine structure of auxospores in a Chaetoceros species isolated from the Acadian coast of New Brunswick, Canada. Auxospore development in this species occurs in a terminal rather than lateral position, a characteristic never before observed in this genus. Our observations suggest that auxosporulation was uniparental, probably an extreme form of autogamy with sister nuclei fusing following meiosis II. Mature auxospores were adze-shaped to sub-globular and contained both scales and transverse perizonia in their walls. The transverse perizonial band structure was similar to longitudinal perizonial bands found in other species of Chaetoceros and differed from the pinnate bands of pennate transverse perizonia, which consisted of a central rib and bilateral fimbria. Instead, the band structure in C. acadianus was more similar to unilateral fimbriate bands in cymatosiroids. We also propose that our diatom represents a species new to science and is a member of the Chaetoceros Section Compressa. We provide its morphological, molecular and reproductive characterization.

Additional file 1: Table S1. Genomes and transcriptomes of diplonemid, euglenid and kinetoplstid species analyzed in this study. Naegleria gruberi was used as an outgroup in phylogenomic analysis. Table S2. Genomes and transcriptomes of free-living and parasitic/symbiotic protists used in this study.