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Additional file 23. Microarray image data from replicate 3 of MCF-7 breast cancer cells grown in static culture.

Additional file 12: Table S1. Total number of ones, zeros and nulls in the sparse matrix for the 163 TFs used in this study.

A video showing the flash display game

Additional file 2: Table S1. Raw data used for all statistical analyses.

Additional file 3 : Table S2. Exact file IDs and links to all data obtained from ENCODE and Roadmap Epigenome.

Electronic Supplementary Material including: - Zircon U-Pb/Hf/O analyses of unknowns and calibration+validation material - Supplementary diagrams for data quality check - CL+BSE images of analyzed grains

Additional file 4. HBD3 28AA analogue characterization. Panels A shows MALDI-TOF of crude 28AA analogue, showing [M + H] + peak (found 3386.4, calculated. 3387). m/z is mass to charge ratio and Intensity is relative abundance or signal intensity of the ions.

Additional file 1: Supplementary Figure S1. Heatmap representing the bacterial community composition in each sample determined using 16S rRNA gene (D), 16S rRNA (R) and 16S rRNA reads recovered from metagenomic data (M). Results of the two duplicate samples are shown for 16S rRNA gene and 16s rRNA. Only lineages with relative abundance >1% in at least one sample are shown. The right part of the graph represents the bacterial 16S rRNA gene quantification in the duplicate samples (blue bars), as well as the relative proportion between bacteria and archaea determined by 16S rRNA gene qPCR quantification (D; red bars) and in the metagenomic dataset (M; purple bar).

During spawning, adult Pacific salmonids (Oncorhynchus spp.) complete challenging upriver migrations during which energy and oxygen delivery must be partitioned into activities such as locomotion, maturation and spawning behaviours under the constraints of an individual's cardiac capacity. To advance our understanding of cardiac function in free-swimming fishes, we implanted migrating adult Chinook salmon (Oncorhynchus tshawytscha) collected near the mouth of the Sydenham River, Ontario, with heart rate (fH) biologgers that recorded fH every 3 min until these semelparous fish expired on spawning grounds several days later. Fundamental aspects of cardiac function were quantified, including resting, routine and maximum fH, as well as scope for fH (maximum−resting fH). Predictors of fH were explored using generalized least-squares regression, including water temperature, discharge, fish size and fish origin (wild versus hatchery). Heart rate was positively correlated with water temperature, which aligned closely with daily and seasonal shifts. Wild fish had slower resting heart rates than hatchery fish, which led to significantly higher scope for fH. Our findings suggest that wild salmon may have better cardiac capacity during migration than hatchery fish, potentially promoting migration success in wild fish.This article is part of the theme issue ‘Measuring physiology in free living animals (Part I)’.

Additional file 37. Microarray image data from replicate 1 of SK-BR-3 breast cancer cells exposed to fluid flow in a parallel-plate bioreactor system.