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Nucleotide sequence of gBlock2 containing kanamycin resistance gene. Black text: Kanamycin resistance gene from pKT25. Red text: Sequence overlapping with pFCF1 region upstream of NcoI restriction endonuclease site. Green text: Sequence overlapping with pFCF1 region downstream of ScaI restriction endonuclease site. Orange text: Region encoding the HA tag. Start codon is highlighted in yellow. (TIF 320 kb)

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Additional file 1. Appendix

Tim Louis is a practicing lawyer who has been actively involved in Vancouver civic politics for over forty years. Tim has lived and worked in Vancouver all his adult life. From his early days as a law student at UBC – during which time he co-founded Vancouver’s custom transit service for people with disabilities [HandyDART], through to his terms as an elected official on both Park Board and City Council, Tim has listened to, and advocated on behalf of, communities across Vancouver. Penny Parry was born in Montreal and moved to BC in the late 1970’s. She has worked as a university professor, psychologist and artist, among other things. She is a former Child and Youth Advocate for the City of Vancouver. Pennyhas a BFA from Emily Carr University of Art + Design, and a BA and PhD in psychology.

Simulation of a black-hole binary system evolved by the SpEC code.

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View of north end from the east side during construction.

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