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116 Research products, page 1 of 12

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  • Research data . 2017
    Open Access
    Authors: 
    Mazo, Alexander;
    Project: CIHR

    Original images associated with MOLECULAR-CELL-D-16-01448R2.

  • Open Access
    Authors: 
    Fudge, Neva J.; Mearow, Karen M.;
    Publisher: Dryad
    Project: CIHR , NSERC

    Background: In our previous investigations of the role of the extracellular matrix (ECM) in promoting neurite growth we have observed that a permissive laminin (LN) substrate stimulates differential growth responses in subpopulations of mature dorsal root ganglion (DRG) neurons. DRG neurons expressing Trk and p75 receptors grow neurites on a LN substrate in the absence of neurotrophins, while isolectin B4-binding neurons (IB4+) do not display significant growth under the same conditions. We set out to determine whether there was an expression signature of the LN-induced neurite growth phenotype. Using a lectin binding protocol IB4+ neurons were isolated from dissociated DRG neurons, creating two groups - IB4+ and IB4-. A small-scale microarray approach was employed to screen the expression of a panel of ECM-associated genes following dissociation (t=0) and after 24 hr culture on LN (t=24LN). This was followed by qRT-PCR and immunocytochemistry of selected genes. Results: The microarray screen showed that 36 of the 144 genes on the arrays were consistently expressed by the neurons. The array analyses showed that six genes had lower expression in the IB4+ neurons compared to the IB4- cells at t=0 (CTSH, Icam1, Itgβ1, Lamb1, Plat, Spp1), and one gene was expressed at higher levels in the IB4+ cells (Plaur). qRT-PCR was carried out as an independent assessment of the array results. There were discrepancies between the two methods, with qRT-PCR confirming the differences in Lamb1, Plat and Plaur, and showing decreased expression of AdamTs1, FN, and Icam in the IB4+ cells at t=0. After 24 hr culture on LN, there were no significant differences detected by qRT-PCR between the IB4+ and IB4- cells. However, both groups showed upregulation of Itgβ1 and Plaur after 24 hr on LN, the IB4+ group also had increased Plat, and the IB4- cells showed decreased Lamb1, Icam1 and AdamTs1. Further, the array screen also detected a number of genes (not subjected to qRT-PCR) expressed similarly by both populations in relatively high levels but not detectably influenced by time in culture (Bsg, Cst3, Ctsb, Ctsd, Ctsl, Mmp14, Mmp19, Sparc. We carried out immunohistochemistry to confirm expression of proteins encoded by a number of these genes. Conclusions:Our results show that 1B4+ and IB4- neurons differ in the expression of several genes that are associated with responsiveness to the ECM prior to culturing (AdamTs1, FN, Icam1, Lamb1, Plat, Plaur). The data suggest that the genes expressed at higher levels in the IB4- neurons could contribute to the initial growth response of these cells in a permissive environment and could also represent a common injury response that subsequently promotes axon regeneration. The differential expression of several extracellular matrix molecules (FN, Lamb1, Icam) may suggest that the IB4- neurons are capable of maintaining /secreting their local extracellular environment which could aid in the regenerative process. Overall, these data provide new information on potential targets that could be manipulated to enhance axonal regeneration in the mature nervous system.

  • Research data . 2017
    Open Access
    Authors: 
    Mazo, Alexander;
    Project: CIHR

    Original images associated with MOLECULAR-CELL-D-16-01448R2.

  • Open Access
    Authors: 
    Lambert, J (via Mendeley Data);
    Publisher: Mendeley
    Project: NSERC

    U-2 OS cells were seeded onto 8-well LabTek II imaging chambers (ThermoFisher) in complete media with 1 μg/mL tetracycline and grown for 24 h to allow expression of GFP-tagged protein. Before imaging, the media was replaced with 200 μL of phenol red-free DMEM supplemented with 10% FBS, 1 mM sodium pyruvate, and 1× Glutamax (all Life Technologies). Images stacks were acquired on an imaging system (DeltaVision Elite, GE Healthcare). Cells were imaged at 37°C in 5% CO2 at 60×, 1.42NA, with 2×2 binning. Image Z-stacks of 24 μm were aquired at 2 μm intervals over 2 - 5 min as indicated. 20 s after the start of data acquisition, 100 μL of warm media containing 1.5 μM JQ1 was added to each cell chamber manually for a final concentration of 500 nM. The exposure time was 10 ms at 32% for GFP-tagged bait protein. Z-stacks were collected, deconvolved using softWoRx (v5.0, Applied Precision) and displayed as maximum intensity projections (pixel size 0.1075 μm). Images were cropped in ImageJ (National Institutes of Health). Supplemental Movie S1 – Flp-In T-REx U-2 OS cells expressing GFP-tagged BRD2 treated with 500 nM JQ1 for 2 min. Supplemental Movie S2 – Flp-In T-REx U-2 OS cells expressing GFP-tagged BRD3 (WT construct) treated with 500 nM JQ1 for 2 min. Supplemental Movie S3 – Flp-In T-REx U-2 OS cells expressing GFP-tagged BRD4 treated with 500 nM JQ1 for 2 min. Supplemental Movie S4 – Flp-In T-REx U-2 OS cells expressing GFP-tagged BRD3 BD1mut treated with 500 nM JQ1 for 2 min. Supplemental Movie S5 – Flp-In T-REx U-2 OS cells expressing GFP-tagged BRD3 BD2mut treated with 500 nM JQ1 for 2 min. Supplemental Movie S6 – Flp-In T-REx U-2 OS cells expressing GFP-tagged BRD3 (BD1:2)mut treated with 500 nM JQ1 for 2 min.

  • Open Access
    Authors: 
    Lee, J (via Mendeley Data);
    Publisher: Data Archiving and Networked Services (DANS)
    Project: CIHR

    Flow cytometric analyses of day 4 CD235a/Aldefluor and day20 MLC2V/CTNT expression of HES2 hESC (Figure S8) and MSC-iPS1 hiPSC lines (Figure S9)

  • Open Access
    Authors: 
    Yousefi, Maryam;
    Publisher: Mendeley
    Project: NSERC

    The two spreadsheets present the contribution of Glacial Isostatic Adjustment (GIA) to present-day vertical and horizontal land motion at 483 GPS stations and also sea-level change at 56 tide gauge stations along the Pacific coast of central North America.

  • Open Access
    Authors: 
    Lamaze, Fabien C.; Pavey, Scott A.; Normandeau, Eric; Roy, Gabriel; Garant, Dany; Bernatchez, Louis;
    Publisher: Data Archiving and Networked Services (DANS)
    Project: NSERC

    2009 dataThis dataset contains: gene expression values, physiological parameters, Q_values, Minisatellites length, MHCIIb alleles, parasitological parameters.data_2009_MEC-13-1125.xlsx2008-2009_parasites_dataThis dataset contains parasitological informations for the individuals sampled in 2008 and 2009. The lakes, refere to Amanites (AMA), Belles de jour (BEL), Caribou (Car), Méthot (MET), Main de fer (MAI) and Petit Saint-Bernard (BER).data_2008-2009_parasites_MEC-13-1125.xlsxMHCIIb_genotypes_dataThis dataset was generated with the genotyping pipeline described in the manuscript. It contains MHCIIb genotypes for all populations. Lakes refere to : Amanites (AMA), Belles-de-Jour (BEL), Methot (MET), Arcand (ARC), Rivard (RIV), Veillette (VEI), Caribou (CAR), Main de fer (MAI), Sorbier (SOR) and Petit saint Bernard (BER).data_genotype_2008_MHCII.xlsxData of the laboratory experimentThis dataset contains the following variables: MHCIIb gene expression values, physiological parameters (weight, length and Fulton condition index), Minisatellites groups (either long or short based on the number of repeat), family and temperature.data_laboratory-experiment.xlsx454 raw MHC2b sequencesThis file contains raw 454 sequences of the MHC2b gene.454_seq_2011090210000039.zipMHC_alleles_sequencesSequences of the 29 MHC2b alleles described in the study. These sequences were aligned with muscle, using MEGAv5 and are in the fasta format.MHC_alleles_aligned_10-04-2012.fasta The capacity of an individual to battle infection is an important fitness determinant in wild vertebrate populations. The major histocompatibility complex (MHC) genes are crucial for a host’s adaptive immune system to detect pathogens. However, anthropogenic activities may disrupt natural cycles of co-evolution between hosts and pathogens. In this study we investigated the dynamic sequence and expression variation of host parasite interactions in brook charr (Salvelinus fontinalis) in a context of past human disturbance via population supplementation from domestic individuals. To do so, we developed a new method to examine selection shaping MHC diversity within and between populations and found a complex interplay between neutral and selective processes that varied among lakes that were investigated. We provided evidence for a lower introgression rate of domestic alleles and found that parasite infection increased with domestic genomic background of individuals. We also documented an association between individual MHC alleles and parasite taxa. Finally, longer cis regulatory minisatellites were positively correlated with MHC II down-regulation and domestic admixture, suggesting that inadvertent selection during domestication resulted in a lower immune response capacity, through a trade-off between growth and immunity, which explained the negative selection of domestic alleles at least under certain circumstances.

  • Open Access
    Authors: 
    Quesnel-Vallières, M;
    Publisher: Mendeley
    Project: CIHR , EC | NEURAL AS (637591)

    Primary image data related to Quesnel-Vallières et al., 2016, Mol Cell: http://dx.doi.org/10.1016/j.molcel.2016.11.033

  • Open Access
    Authors: 
    Chu, Jackson W. F.; Gale, Katie S. P.;
    Publisher: Data Archiving and Networked Services (DANS)
    Project: NSERC

    Expansion of oxygen deficient waters (hypoxia) in the northeast Pacific Ocean (NEP) will have marked impacts on marine life. The response of the resident communities will be a function of their ecophysiological constraints in low oxygen, although this remains untested in the NEP due to a lack of integrative studies. Here, we combine in situ surveys and lab-based respirometry experiments were conducted on three indicator species (spot prawn Pandalus platyceros, slender sole Lyopsetta exilis, squat lobster Munida quadrispina) of seasonally hypoxic systems in the NEP to test if metabolic constraints determine distributions and energy sequestration in a hypoxic setting. These experiments were integrated with a global review of critical oxygen levels ( math formula; lower threshold of aerobic metabolism) for crustaceans to determine if math formula-based hypoxia thresholds are different among ocean basins. Our results show that species-specific differences in math formula and standard metabolic rates (1) determine the lowest environmental oxygen ([O2]env) at which in situ populations occur, (2) result in disproportionate shifts in distributions among co-occurring species during summer hypoxia expansion events, and (3) characterize shifts in megafaunal community respiration rates due to marked spatio-temporal variability in [O2]env. Our results show that math formula-based hypoxia thresholds are significantly lower in the East Pacific Ocean relative to other major ocean basins, which suggests that the physiological response of local fauna to deoxygenation can be determined by the natural variability and oxygen exposure in a region. In order to establish realistic predictions on the biological consequences of marine deoxygenation, we suggest integrating metabolism-based traits to calculate hypoxia thresholds for marine ecosystems. CHONe_EF-13_ChuJ_Data_LnO2016Data are from lab-based respirometry experiments designed to measure metabolic rates (oxygen consumption) and critical oxygen tensions (O2crit or Pcrit) for slender sole, spot prawn, and squat lobster. Data was collected as part of a PhD thesis (J.W.F. Chu).

  • Open Access
    Authors: 
    John Ballantyne;
    Publisher: Data Archiving and Networked Services (DANS)
    Project: NSERC

    Electric charging is one of the essential aerosol dynamic mechanisms and is harnessed for detection, capture and control of ultrafine aerosol particles in a range of devices. For simplicity, charging and transport mechanisms are commonly modelled with zero spatial dimensions (0-D) and averaged properties such as mean charge or mean particle diameter. These models often neglect localised effects of the flow distribution, diffusion, discrete charge states, and particle polydispersity, often proving inadequate to explain experimental data. This work aims to provide an open-source three-dimensional (3-D) aerosol charging and transport model including bipolar and unipolar diffusion charging, and photoelectric charging algorithms for use in detailed design and analyses of aerosol systems. The computational model consists of more than 200 particle transport equations for discrete charge states and polydisperse sizes coupled with ion conservation equations in the framework of OpenFOAM, an open-source computational fluid dynamics platform. Three test cases are introduced to verify implementation of three charging models by comparison with published literature: bipolar and unipolar diffusion charging, and photoelectric charging. Tutorial cases, which model three distinct aerosol sensors, are described and demonstrate the capabilities of the 3-D aerosol charging and transport models within the predetermined flow field. The aerosolChargingFoam code is available at https://openaerosol.sourceforge.io for widespread use and can be further modified under the GNU general public licence. THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOVE

search
Include:
The following results are related to Canada. Are you interested to view more results? Visit OpenAIRE - Explore.
116 Research products, page 1 of 12
  • Research data . 2017
    Open Access
    Authors: 
    Mazo, Alexander;
    Project: CIHR

    Original images associated with MOLECULAR-CELL-D-16-01448R2.

  • Open Access
    Authors: 
    Fudge, Neva J.; Mearow, Karen M.;
    Publisher: Dryad
    Project: CIHR , NSERC

    Background: In our previous investigations of the role of the extracellular matrix (ECM) in promoting neurite growth we have observed that a permissive laminin (LN) substrate stimulates differential growth responses in subpopulations of mature dorsal root ganglion (DRG) neurons. DRG neurons expressing Trk and p75 receptors grow neurites on a LN substrate in the absence of neurotrophins, while isolectin B4-binding neurons (IB4+) do not display significant growth under the same conditions. We set out to determine whether there was an expression signature of the LN-induced neurite growth phenotype. Using a lectin binding protocol IB4+ neurons were isolated from dissociated DRG neurons, creating two groups - IB4+ and IB4-. A small-scale microarray approach was employed to screen the expression of a panel of ECM-associated genes following dissociation (t=0) and after 24 hr culture on LN (t=24LN). This was followed by qRT-PCR and immunocytochemistry of selected genes. Results: The microarray screen showed that 36 of the 144 genes on the arrays were consistently expressed by the neurons. The array analyses showed that six genes had lower expression in the IB4+ neurons compared to the IB4- cells at t=0 (CTSH, Icam1, Itgβ1, Lamb1, Plat, Spp1), and one gene was expressed at higher levels in the IB4+ cells (Plaur). qRT-PCR was carried out as an independent assessment of the array results. There were discrepancies between the two methods, with qRT-PCR confirming the differences in Lamb1, Plat and Plaur, and showing decreased expression of AdamTs1, FN, and Icam in the IB4+ cells at t=0. After 24 hr culture on LN, there were no significant differences detected by qRT-PCR between the IB4+ and IB4- cells. However, both groups showed upregulation of Itgβ1 and Plaur after 24 hr on LN, the IB4+ group also had increased Plat, and the IB4- cells showed decreased Lamb1, Icam1 and AdamTs1. Further, the array screen also detected a number of genes (not subjected to qRT-PCR) expressed similarly by both populations in relatively high levels but not detectably influenced by time in culture (Bsg, Cst3, Ctsb, Ctsd, Ctsl, Mmp14, Mmp19, Sparc. We carried out immunohistochemistry to confirm expression of proteins encoded by a number of these genes. Conclusions:Our results show that 1B4+ and IB4- neurons differ in the expression of several genes that are associated with responsiveness to the ECM prior to culturing (AdamTs1, FN, Icam1, Lamb1, Plat, Plaur). The data suggest that the genes expressed at higher levels in the IB4- neurons could contribute to the initial growth response of these cells in a permissive environment and could also represent a common injury response that subsequently promotes axon regeneration. The differential expression of several extracellular matrix molecules (FN, Lamb1, Icam) may suggest that the IB4- neurons are capable of maintaining /secreting their local extracellular environment which could aid in the regenerative process. Overall, these data provide new information on potential targets that could be manipulated to enhance axonal regeneration in the mature nervous system.

  • Research data . 2017
    Open Access
    Authors: 
    Mazo, Alexander;
    Project: CIHR

    Original images associated with MOLECULAR-CELL-D-16-01448R2.

  • Open Access
    Authors: 
    Lambert, J (via Mendeley Data);
    Publisher: Mendeley
    Project: NSERC

    U-2 OS cells were seeded onto 8-well LabTek II imaging chambers (ThermoFisher) in complete media with 1 μg/mL tetracycline and grown for 24 h to allow expression of GFP-tagged protein. Before imaging, the media was replaced with 200 μL of phenol red-free DMEM supplemented with 10% FBS, 1 mM sodium pyruvate, and 1× Glutamax (all Life Technologies). Images stacks were acquired on an imaging system (DeltaVision Elite, GE Healthcare). Cells were imaged at 37°C in 5% CO2 at 60×, 1.42NA, with 2×2 binning. Image Z-stacks of 24 μm were aquired at 2 μm intervals over 2 - 5 min as indicated. 20 s after the start of data acquisition, 100 μL of warm media containing 1.5 μM JQ1 was added to each cell chamber manually for a final concentration of 500 nM. The exposure time was 10 ms at 32% for GFP-tagged bait protein. Z-stacks were collected, deconvolved using softWoRx (v5.0, Applied Precision) and displayed as maximum intensity projections (pixel size 0.1075 μm). Images were cropped in ImageJ (National Institutes of Health). Supplemental Movie S1 – Flp-In T-REx U-2 OS cells expressing GFP-tagged BRD2 treated with 500 nM JQ1 for 2 min. Supplemental Movie S2 – Flp-In T-REx U-2 OS cells expressing GFP-tagged BRD3 (WT construct) treated with 500 nM JQ1 for 2 min. Supplemental Movie S3 – Flp-In T-REx U-2 OS cells expressing GFP-tagged BRD4 treated with 500 nM JQ1 for 2 min. Supplemental Movie S4 – Flp-In T-REx U-2 OS cells expressing GFP-tagged BRD3 BD1mut treated with 500 nM JQ1 for 2 min. Supplemental Movie S5 – Flp-In T-REx U-2 OS cells expressing GFP-tagged BRD3 BD2mut treated with 500 nM JQ1 for 2 min. Supplemental Movie S6 – Flp-In T-REx U-2 OS cells expressing GFP-tagged BRD3 (BD1:2)mut treated with 500 nM JQ1 for 2 min.

  • Open Access
    Authors: 
    Lee, J (via Mendeley Data);
    Publisher: Data Archiving and Networked Services (DANS)
    Project: CIHR

    Flow cytometric analyses of day 4 CD235a/Aldefluor and day20 MLC2V/CTNT expression of HES2 hESC (Figure S8) and MSC-iPS1 hiPSC lines (Figure S9)

  • Open Access
    Authors: 
    Yousefi, Maryam;
    Publisher: Mendeley
    Project: NSERC

    The two spreadsheets present the contribution of Glacial Isostatic Adjustment (GIA) to present-day vertical and horizontal land motion at 483 GPS stations and also sea-level change at 56 tide gauge stations along the Pacific coast of central North America.

  • Open Access
    Authors: 
    Lamaze, Fabien C.; Pavey, Scott A.; Normandeau, Eric; Roy, Gabriel; Garant, Dany; Bernatchez, Louis;
    Publisher: Data Archiving and Networked Services (DANS)
    Project: NSERC

    2009 dataThis dataset contains: gene expression values, physiological parameters, Q_values, Minisatellites length, MHCIIb alleles, parasitological parameters.data_2009_MEC-13-1125.xlsx2008-2009_parasites_dataThis dataset contains parasitological informations for the individuals sampled in 2008 and 2009. The lakes, refere to Amanites (AMA), Belles de jour (BEL), Caribou (Car), Méthot (MET), Main de fer (MAI) and Petit Saint-Bernard (BER).data_2008-2009_parasites_MEC-13-1125.xlsxMHCIIb_genotypes_dataThis dataset was generated with the genotyping pipeline described in the manuscript. It contains MHCIIb genotypes for all populations. Lakes refere to : Amanites (AMA), Belles-de-Jour (BEL), Methot (MET), Arcand (ARC), Rivard (RIV), Veillette (VEI), Caribou (CAR), Main de fer (MAI), Sorbier (SOR) and Petit saint Bernard (BER).data_genotype_2008_MHCII.xlsxData of the laboratory experimentThis dataset contains the following variables: MHCIIb gene expression values, physiological parameters (weight, length and Fulton condition index), Minisatellites groups (either long or short based on the number of repeat), family and temperature.data_laboratory-experiment.xlsx454 raw MHC2b sequencesThis file contains raw 454 sequences of the MHC2b gene.454_seq_2011090210000039.zipMHC_alleles_sequencesSequences of the 29 MHC2b alleles described in the study. These sequences were aligned with muscle, using MEGAv5 and are in the fasta format.MHC_alleles_aligned_10-04-2012.fasta The capacity of an individual to battle infection is an important fitness determinant in wild vertebrate populations. The major histocompatibility complex (MHC) genes are crucial for a host’s adaptive immune system to detect pathogens. However, anthropogenic activities may disrupt natural cycles of co-evolution between hosts and pathogens. In this study we investigated the dynamic sequence and expression variation of host parasite interactions in brook charr (Salvelinus fontinalis) in a context of past human disturbance via population supplementation from domestic individuals. To do so, we developed a new method to examine selection shaping MHC diversity within and between populations and found a complex interplay between neutral and selective processes that varied among lakes that were investigated. We provided evidence for a lower introgression rate of domestic alleles and found that parasite infection increased with domestic genomic background of individuals. We also documented an association between individual MHC alleles and parasite taxa. Finally, longer cis regulatory minisatellites were positively correlated with MHC II down-regulation and domestic admixture, suggesting that inadvertent selection during domestication resulted in a lower immune response capacity, through a trade-off between growth and immunity, which explained the negative selection of domestic alleles at least under certain circumstances.

  • Open Access
    Authors: 
    Quesnel-Vallières, M;
    Publisher: Mendeley
    Project: CIHR , EC | NEURAL AS (637591)

    Primary image data related to Quesnel-Vallières et al., 2016, Mol Cell: http://dx.doi.org/10.1016/j.molcel.2016.11.033

  • Open Access
    Authors: 
    Chu, Jackson W. F.; Gale, Katie S. P.;
    Publisher: Data Archiving and Networked Services (DANS)
    Project: NSERC

    Expansion of oxygen deficient waters (hypoxia) in the northeast Pacific Ocean (NEP) will have marked impacts on marine life. The response of the resident communities will be a function of their ecophysiological constraints in low oxygen, although this remains untested in the NEP due to a lack of integrative studies. Here, we combine in situ surveys and lab-based respirometry experiments were conducted on three indicator species (spot prawn Pandalus platyceros, slender sole Lyopsetta exilis, squat lobster Munida quadrispina) of seasonally hypoxic systems in the NEP to test if metabolic constraints determine distributions and energy sequestration in a hypoxic setting. These experiments were integrated with a global review of critical oxygen levels ( math formula; lower threshold of aerobic metabolism) for crustaceans to determine if math formula-based hypoxia thresholds are different among ocean basins. Our results show that species-specific differences in math formula and standard metabolic rates (1) determine the lowest environmental oxygen ([O2]env) at which in situ populations occur, (2) result in disproportionate shifts in distributions among co-occurring species during summer hypoxia expansion events, and (3) characterize shifts in megafaunal community respiration rates due to marked spatio-temporal variability in [O2]env. Our results show that math formula-based hypoxia thresholds are significantly lower in the East Pacific Ocean relative to other major ocean basins, which suggests that the physiological response of local fauna to deoxygenation can be determined by the natural variability and oxygen exposure in a region. In order to establish realistic predictions on the biological consequences of marine deoxygenation, we suggest integrating metabolism-based traits to calculate hypoxia thresholds for marine ecosystems. CHONe_EF-13_ChuJ_Data_LnO2016Data are from lab-based respirometry experiments designed to measure metabolic rates (oxygen consumption) and critical oxygen tensions (O2crit or Pcrit) for slender sole, spot prawn, and squat lobster. Data was collected as part of a PhD thesis (J.W.F. Chu).

  • Open Access
    Authors: 
    John Ballantyne;
    Publisher: Data Archiving and Networked Services (DANS)
    Project: NSERC

    Electric charging is one of the essential aerosol dynamic mechanisms and is harnessed for detection, capture and control of ultrafine aerosol particles in a range of devices. For simplicity, charging and transport mechanisms are commonly modelled with zero spatial dimensions (0-D) and averaged properties such as mean charge or mean particle diameter. These models often neglect localised effects of the flow distribution, diffusion, discrete charge states, and particle polydispersity, often proving inadequate to explain experimental data. This work aims to provide an open-source three-dimensional (3-D) aerosol charging and transport model including bipolar and unipolar diffusion charging, and photoelectric charging algorithms for use in detailed design and analyses of aerosol systems. The computational model consists of more than 200 particle transport equations for discrete charge states and polydisperse sizes coupled with ion conservation equations in the framework of OpenFOAM, an open-source computational fluid dynamics platform. Three test cases are introduced to verify implementation of three charging models by comparison with published literature: bipolar and unipolar diffusion charging, and photoelectric charging. Tutorial cases, which model three distinct aerosol sensors, are described and demonstrate the capabilities of the 3-D aerosol charging and transport models within the predetermined flow field. The aerosolChargingFoam code is available at https://openaerosol.sourceforge.io for widespread use and can be further modified under the GNU general public licence. THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOVE