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  • Open Access
    Authors: 
    Zizic, Jovana B.; Vukovic, Nenad L.; Jadranin, Milka; Anđelković, Boban D.; Tešević, Vele; Kacaniova, Miroslava M.; Sukdolak, Slobodan B.; Marković, Snežana D.;
    Publisher: Wiley, Hoboken
    Country: Serbia
    Project: MESTD | Preclinical investigation... (41010), MESTD | Natural products of wild,... (172053)

    BACKGROUND Propolis is a complex resinous sticky substance that honeybees collect from buds and exudates of various plants. Owing to its versatile biological and pharmacological activities, propolis is widely used in medicines, cosmetics and foods. The aim of this study was to evaluate the cytotoxic and antioxidative effects of various ethanolic extracts of propolis (EEPs) on human colon cancer cell line HCT-116 and compare them with their composition determined by HPLC-DAD. RESULTS The most abundant flavonoids in all samples were chrysin, pinocembrin and galangin (12.697-40.811 mu gmg(-1)), while the main phenolic acids were caffeic acid, ferulic acid and isoferulic acid. Dose- and time-dependent inhibition of growth of HCT-116 cells was observed for all propolis samples, with IC50 values ranging from 26.33 to 143.09 mu gmL(-1). Differences in cytotoxic activity of propolis samples were associated with differences in their composition. All EEP samples reduced both superoxide anion radical and nitrite levels and also had strong DPPH-scavenging activity. CONCLUSION All tested propolis samples had pronounced cytotoxic and antioxidative activities. This is the peer-reviewed version of the following article: Žižić, J. B.; Vuković, N. L.; Jadranin, M.; Anđelković, B. D.; Tešević, V.; Kacaniova, M. M.; Sukdolak, S. B.; Markovic, S. D. Chemical Composition, Cytotoxic and Antioxidative Activities of Ethanolic Extracts of Propolis on HCT-116 Cell Line. Journal of the Science of Food and Agriculture 2013, 93 (12), 3001–3009. [https://doi.org/10.1002/jsfa.6132] [http://cer.ihtm.bg.ac.rs/handle/123456789/1213]

  • Open Access
    Authors: 
    Katarina Smiljanic; Danijela Apostolovic; Snežana Trifunović; Jana Ognjenovic; Marija Perusko; Luka Mihajlovic; Lidija Burazer; M. van Hage; T. Cirkovic Velickovic;
    Publisher: Blackwell Publishing Ltd
    Countries: Belgium, Serbia, Serbia
    Project: MESTD | Molecular properties and ... (172024), EC | FCUB-ERA (256716)

    Background Short ragweed (Ambrosia artemisiifolia) allergies affect more than 36 million people annually. Ragweed pollen grains release subpollen particles (SPP) of respirable size upon hydration or a change in air electrical conditions. The aim of this study was to characterize the proteomes and allergomes of short ragweed SPP and total pollen protein extract (TOT), and compare their effects with those of standard aqueous pollen protein extract (APE) using sera from short ragweed pollen-sensitized patients. Methods Quantitative 2D gel-based and shotgun proteomics, 1D and 2D immunoblotting, and quantitative ELISA were applied. Novel SPP extraction and preparation protocols enabled appropriate sample preparation and further downstream analysis by quantitative proteomics. Results The SPP fraction contained the highest proportion (94%) of the allergome, with the largest quantities of the minor Amb a 4 and major Amb a 1 allergens, and as unique, NADH dehydrogenases. APE was the richest in Amb a 6, Amb a 5 and Amb a 3, and TOT fraction was the richest in the Amb a 8 allergens (89% and 83% of allergome, respectively). Allergenic potency correlated well among the three fractions tested, with 1D immunoblots demonstrating a slight predominance of IgE reactivity to SPP compared to TOT and APE. However, the strongest IgE binding in ELISA was noted against APE. New allergenic candidates, phosphoglycerate mutase and phosphoglucomutase, were identified in all the three pollen fractions. Enolase, UTP-glucose-1-phosphate uridylyltransferase and polygalacturonase were observed in SPP and TOT fractions as novel allergens of the short ragweed pollen, as previously described. Conclusion and Clinical Relevance We demonstrated that the complete major (Amb a 1 and 11) and almost all minor (Amb a 3, 4, 5, 6, 8 and 9) short ragweed pollen allergen repertoire as well as NADH oxidases are present in SPP, highlighting an important role for SPP in allergic sensitization to short ragweed. This is the peer-reviewed version of the following article: Smiljanic, K.; Apostolovic, D.; Trifunovic, S.; Ognjenovic, J.; Perusko, M.; Mihajlovic, L.; Burazer, L.; van Hage, M.; Cirkovic Velickovic, T. Subpollen Particles Are Rich Carriers of Major Short Ragweed Allergens and NADH Dehydrogenases: Quantitative Proteomic and Allergomic Study. Clinical and Experimental Allergy 2017, 47 (6), 815–828. [https://doi.org/10.1111/cea.12874] Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/3127]

  • Open Access
    Authors: 
    Goran N. Kaludjerović; Frank W. Heinemann; Vukadin M. Leovac; Srećko R. Trifunović; Tibor J. Sabo;
    Publisher: International Union of Crystallography (IUCr)
    Country: Serbia

    In the anion of the title compound, (C2H10N2)[VO(H2O)(C3H2O4)(2)] or H(2)en[VO(mal)(2)H2O], vanadium(IV) is distorted-octahedrally coordinated by six donor O atoms. The two malonate ligands are situated in the equatorial plane, whereas the oxo and the water ligand occupy axial positions. The apical V=O bond exhibits a strong trans influence. The anion possesses crystallographically imposed C-2 symmetry, with the central V atom, the oxo and the water ligand lying on the twofold axis.

  • Open Access
    Authors: 
    Milenković, Milica R.; Papastavrou, Agyro T.; Radanović, Dušanka D.; Pevec, Andrej; Jagličić, Zvonko; Zlatar, Matija; Gruden-Pavlović, Maja; Vougioukalakis, Georgios C.; Turel, Iztok; Anđelković, Katarina K.; +1 more
    Publisher: Elsevier
    Country: Serbia
    Project: MESTD | Interactions of natural p... (172055)

    Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/2865] Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2858] Supplementary material for: [https://www.sciencedirect.com/science/article/pii/S0277538719301664?via%3Dihub]

  • Open Access
    Authors: 
    Živorad Čeković; Radomir N. Saicic;
    Country: Serbia

    Abstract Alkenyl radical generated by β-fragmentation of tertiary cyclohexyloxy radical with carbocyclic ring opening, possessing a suitably located olefinic double bond, undergoes to the intramolecular 5-exo-trigonal cyclization and a new carbocyclic ring was formed.

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Include:
The following results are related to Canada. Are you interested to view more results? Visit OpenAIRE - Explore.
5 Research products, page 1 of 1
  • Open Access
    Authors: 
    Zizic, Jovana B.; Vukovic, Nenad L.; Jadranin, Milka; Anđelković, Boban D.; Tešević, Vele; Kacaniova, Miroslava M.; Sukdolak, Slobodan B.; Marković, Snežana D.;
    Publisher: Wiley, Hoboken
    Country: Serbia
    Project: MESTD | Preclinical investigation... (41010), MESTD | Natural products of wild,... (172053)

    BACKGROUND Propolis is a complex resinous sticky substance that honeybees collect from buds and exudates of various plants. Owing to its versatile biological and pharmacological activities, propolis is widely used in medicines, cosmetics and foods. The aim of this study was to evaluate the cytotoxic and antioxidative effects of various ethanolic extracts of propolis (EEPs) on human colon cancer cell line HCT-116 and compare them with their composition determined by HPLC-DAD. RESULTS The most abundant flavonoids in all samples were chrysin, pinocembrin and galangin (12.697-40.811 mu gmg(-1)), while the main phenolic acids were caffeic acid, ferulic acid and isoferulic acid. Dose- and time-dependent inhibition of growth of HCT-116 cells was observed for all propolis samples, with IC50 values ranging from 26.33 to 143.09 mu gmL(-1). Differences in cytotoxic activity of propolis samples were associated with differences in their composition. All EEP samples reduced both superoxide anion radical and nitrite levels and also had strong DPPH-scavenging activity. CONCLUSION All tested propolis samples had pronounced cytotoxic and antioxidative activities. This is the peer-reviewed version of the following article: Žižić, J. B.; Vuković, N. L.; Jadranin, M.; Anđelković, B. D.; Tešević, V.; Kacaniova, M. M.; Sukdolak, S. B.; Markovic, S. D. Chemical Composition, Cytotoxic and Antioxidative Activities of Ethanolic Extracts of Propolis on HCT-116 Cell Line. Journal of the Science of Food and Agriculture 2013, 93 (12), 3001–3009. [https://doi.org/10.1002/jsfa.6132] [http://cer.ihtm.bg.ac.rs/handle/123456789/1213]

  • Open Access
    Authors: 
    Katarina Smiljanic; Danijela Apostolovic; Snežana Trifunović; Jana Ognjenovic; Marija Perusko; Luka Mihajlovic; Lidija Burazer; M. van Hage; T. Cirkovic Velickovic;
    Publisher: Blackwell Publishing Ltd
    Countries: Belgium, Serbia, Serbia
    Project: MESTD | Molecular properties and ... (172024), EC | FCUB-ERA (256716)

    Background Short ragweed (Ambrosia artemisiifolia) allergies affect more than 36 million people annually. Ragweed pollen grains release subpollen particles (SPP) of respirable size upon hydration or a change in air electrical conditions. The aim of this study was to characterize the proteomes and allergomes of short ragweed SPP and total pollen protein extract (TOT), and compare their effects with those of standard aqueous pollen protein extract (APE) using sera from short ragweed pollen-sensitized patients. Methods Quantitative 2D gel-based and shotgun proteomics, 1D and 2D immunoblotting, and quantitative ELISA were applied. Novel SPP extraction and preparation protocols enabled appropriate sample preparation and further downstream analysis by quantitative proteomics. Results The SPP fraction contained the highest proportion (94%) of the allergome, with the largest quantities of the minor Amb a 4 and major Amb a 1 allergens, and as unique, NADH dehydrogenases. APE was the richest in Amb a 6, Amb a 5 and Amb a 3, and TOT fraction was the richest in the Amb a 8 allergens (89% and 83% of allergome, respectively). Allergenic potency correlated well among the three fractions tested, with 1D immunoblots demonstrating a slight predominance of IgE reactivity to SPP compared to TOT and APE. However, the strongest IgE binding in ELISA was noted against APE. New allergenic candidates, phosphoglycerate mutase and phosphoglucomutase, were identified in all the three pollen fractions. Enolase, UTP-glucose-1-phosphate uridylyltransferase and polygalacturonase were observed in SPP and TOT fractions as novel allergens of the short ragweed pollen, as previously described. Conclusion and Clinical Relevance We demonstrated that the complete major (Amb a 1 and 11) and almost all minor (Amb a 3, 4, 5, 6, 8 and 9) short ragweed pollen allergen repertoire as well as NADH oxidases are present in SPP, highlighting an important role for SPP in allergic sensitization to short ragweed. This is the peer-reviewed version of the following article: Smiljanic, K.; Apostolovic, D.; Trifunovic, S.; Ognjenovic, J.; Perusko, M.; Mihajlovic, L.; Burazer, L.; van Hage, M.; Cirkovic Velickovic, T. Subpollen Particles Are Rich Carriers of Major Short Ragweed Allergens and NADH Dehydrogenases: Quantitative Proteomic and Allergomic Study. Clinical and Experimental Allergy 2017, 47 (6), 815–828. [https://doi.org/10.1111/cea.12874] Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/3127]

  • Open Access
    Authors: 
    Goran N. Kaludjerović; Frank W. Heinemann; Vukadin M. Leovac; Srećko R. Trifunović; Tibor J. Sabo;
    Publisher: International Union of Crystallography (IUCr)
    Country: Serbia

    In the anion of the title compound, (C2H10N2)[VO(H2O)(C3H2O4)(2)] or H(2)en[VO(mal)(2)H2O], vanadium(IV) is distorted-octahedrally coordinated by six donor O atoms. The two malonate ligands are situated in the equatorial plane, whereas the oxo and the water ligand occupy axial positions. The apical V=O bond exhibits a strong trans influence. The anion possesses crystallographically imposed C-2 symmetry, with the central V atom, the oxo and the water ligand lying on the twofold axis.

  • Open Access
    Authors: 
    Milenković, Milica R.; Papastavrou, Agyro T.; Radanović, Dušanka D.; Pevec, Andrej; Jagličić, Zvonko; Zlatar, Matija; Gruden-Pavlović, Maja; Vougioukalakis, Georgios C.; Turel, Iztok; Anđelković, Katarina K.; +1 more
    Publisher: Elsevier
    Country: Serbia
    Project: MESTD | Interactions of natural p... (172055)

    Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/2865] Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2858] Supplementary material for: [https://www.sciencedirect.com/science/article/pii/S0277538719301664?via%3Dihub]

  • Open Access
    Authors: 
    Živorad Čeković; Radomir N. Saicic;
    Country: Serbia

    Abstract Alkenyl radical generated by β-fragmentation of tertiary cyclohexyloxy radical with carbocyclic ring opening, possessing a suitably located olefinic double bond, undergoes to the intramolecular 5-exo-trigonal cyclization and a new carbocyclic ring was formed.