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Ministry of Health Welfare and Sport
Country: Netherlands
16 Projects, page 1 of 4
  • Open Access mandate for Publications
    Funder: EC Project Code: 796009
    Overall Budget: 177,599 EURFunder Contribution: 177,599 EUR
    Partners: VWS

    The adjuvant potential of bacterial lipopolysaccharide (LPS) for vaccines depends on a detailed understanding of its innate immune signalling properties, in order to achieve the right balance between immunogenicity and reactogenicity. By biosynthetic engineering of meningococcal LPS, we have obtained a unique panel of mutants covering a wide range of endotoxic activity, from the maximum wildtype level to almost totally inactive. However, this is based on activation of the TLR4/MD-2 receptor, and recent studies have revealed that LPS endotoxic activity is also mediated by the intracellular receptor caspase 4 (caspase 11 in the mouse). At present, it is not clear how this contributes to the overall biological activity of LPS, and how it can be modified by structural alterations to the lipid A moiety of LPS. A major contribution of the proposed project will be to provide for the first time a detailed structure-function relationship for this LPS-caspase 4/11 interaction. This information will be crucial in the further evaluation of the adjuvant potential of these LPS derivatives, as well as giving more insight in the role of LPS modification in the interaction of pathogenic bacteria with the innate immune system. LPS mutants with differential effects on the TLR4 and caspase pathways will provide a unique opportunity to tease apart the contributions of both pathways to the overall LPS biological activity. In addition, we will explore the role of delivery of LPS by OMVs (outer membrane vesicles) to the intracellular pathway activation. A second and complementary contribution will be the development of novel LPS purification methods, which will also lead to better understanding of LPS structure-function relationships by allowing the separation of complicated LPS mixtures in their individual molecular species. In addition, they will pave the way for purification procedures which can be upscaled for making clinical grade LPS in human vaccine applications.

  • Open Access mandate for Publications
    Funder: EC Project Code: 731116
    Overall Budget: 342,020 EURFunder Contribution: 300,000 EUR
    Partners: VWS

    The Dutch Ministry of Health, Welfare and Sport will organize an international conference focusing on the implementation eHealth in health systems and the use by citizens. This conference will be organized in joint cooperation with the European Commission and aligned with the European conference of the Healthcare Information and Management Systems Society, and together is called the 'eHealth Week 2016'. It is scheduled 6-10 of June 2016 in Amsterdam, The Netherlands.

  • Funder: EC Project Code: 252934
    Partners: VWS, RIVM
  • Funder: EC Project Code: 262208
    Partners: KUL, UL, UPF, VWS, NSD - NORSK SENTER FOR FORSKNINGSDATA AS, City, University of London, GESIS
  • Funder: EC Project Code: 201169
    Partners: LUMC, NIHE, ISTITUTO SUPERIORE DI SANITA, IRB, RIVM, THL, VWS, Fondazione Andrea Cesalpino