CYPROTEX DISCOVERY LIMITED
CYPROTEX DISCOVERY LIMITED
2 Projects, page 1 of 1
Open Access Mandate for Publications and Research data assignment_turned_in Project2019 - 2024 CYPROTEX DISCOVERY LIMITED, UNIVERSITA DEGLI STUDI DI PARMA, Atturos, UM, ULB +4 partnersCYPROTEX DISCOVERY LIMITED,UNIVERSITA DEGLI STUDI DI PARMA,Atturos,UM,ULB,UCD,CNR,ARTTIC,PreSens Precision Sensing (Germany)Funder: EC Project Code: 825745Overall Budget: 5,655,090 EURFunder Contribution: 5,655,090 EURSCREENED will develop 3 different three-dimensional (3D) in vitro assays based on rodent and human thyroid cell constructs. At different level of anatomical complexity, these assays will increasingly mimic the structure and function of the native thyroid gland, ultimately achieving the replication of its vascular anatomy and morphogenetic characteristics: 1) a 3D organoid based on stem-cell derived thyroid cells, 2) a decellularized scaffold able to reproduce the biological composition of a native thyroid, repopulated by the 3D organoids, and 3) a bioprinted construct with the 3D organoids able to mimic the spatial and geometrical architecture of a native thyroid. These 3D assays will be hosted in a modular microbioreactor equipped with innovative sensing technology and enabling precise control of cell culture conditions. New superparamagnetic biocompatible and biomimetic particles will be used to produce “magnetic cells” to support precise spatiotemporal homing of the cells in the 3D decellularized and bioprinted constructs. The 3D assays will be used to screen the effect of endocrine disruptors (EDs) on the thyroid function in a unique biological sex-specific approach. Their performance will be assessed individually, in comparison with each other, and against in vivo studies. The 3D assays are expected to predict with more sensitivity and specificity the thyroid responses to different classes of EDs at low doses, compared to classical 2D in vitro assays or animal models. Supporting the “Adverse Outcome Pathway” concept, proteogenomics analysis and biological modelling of the underlying mode of action will be used to gain a mechanistic understanding of the chain of events from exposure to adverse effects on thyroid development and function. For future uptake, SCREENED will engage discussion with relevant stakeholder groups, including regulatory bodies and industry, to ensure that the assays are fit for purpose for ED safety assessment
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For further information contact us at helpdesk@openaire.euOpen Access Mandate for Publications assignment_turned_in Project2016 - 2021 Leiden University, KI, CYPROTEX DISCOVERY LIMITED, HULAFE, Roche (Switzerland) +39 partnersLeiden University,KI,CYPROTEX DISCOVERY LIMITED,HULAFE,Roche (Switzerland),ISTITUTO SUPERIORE DI SANITA,IRFMN,EwC,UCPH,BASF SE,DEPA,DTU,INSPHERO,FHG,BDS,University of Konstanz,KUL,Heidelberg University,UM,L'OREAL SA,ARTTIC,University of Vienna,OPF,RISE,BAuA,UKRI,IFADO,MUI,VUA,MRC,INSTITUT NATIONAL DE L ENVIRONNEMENT INDUSTRIEL ET DES RISQUES - INERIS,CERTARA UK LIMITED,LL,COSMETICS EUROPE,UNILEVER U.K. CENTRAL RESOURCES LIMITED,BIOT,Stockholm University,Leiden University Medical Center,JHU,TISSUSE GMBH,EMBL,UPF,TNO,StCFunder: EC Project Code: 681002Overall Budget: 30,258,400 EURFunder Contribution: 27,798,300 EURThe vision of EU-ToxRisk is to drive a paradigm shift in toxicology towards an animal-free, mechanism-based integrated approach to chemical safety assessment. The project will unite all relevant disciplines and stakeholders to establish: i) pragmatic, solid read-across procedures incorporating mechanistic and toxicokinetic knowledge; and ii) ab initio hazard and risk assessment strategies of chemicals with little background information. The project will focus on repeated dose systemic toxicity (liver, kidney, lung and nervous system) as well as developmental/reproduction toxicity. Different human tiered test systems are integrated to balance speed, cost and biological complexity. EU-ToxRisk extensively integrates the adverse outcome pathway (AOP)-based toxicity testing concept. Therefore, advanced technologies, including high throughput transcriptomics, RNA interference, and high throughput microscopy, will provide quantitative and mechanistic underpinning of AOPs and key events (KE). The project combines in silico tools and in vitro assays by computational modelling approaches to provide quantitative data on the activation of KE of AOP. This information, together with detailed toxicokinetics data, and in vitro-in vivo extrapolation algorithms forms the basis for improved hazard and risk assessment. The EU-ToxRisk work plan is structured along a broad spectrum of case studies, driven by the cosmetics, (agro)-chemical, pharma industry together with regulators. The approach involves iterative training, testing, optimization and validation phases to establish fit-for-purpose integrated approaches to testing and assessment with key EU-ToxRisk methodologies. The test systems will be combined to a flexible service package for exploitation and continued impact across industry sectors and regulatory application. The proof-of-concept for the new mechanism-based testing strategy will make EU-ToxRisk the flagship in Europe for animal-free chemical safety assessment.
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