Introduction: Active transportation (AT) has dual benefits: population health benefits with increased physical activity and environmental sustainability with decreased carbon emissions. Despite the city’s low geographic density, residents of Kingston, Canada use AT for 9.5% of trips, compared to the country’s average of 6.9%. Additionally, employees of Queen’s University demonstrated even higher rates of active commuting before the COVID-19 pandemic. The objective of this study was to compare the levels and correlates of transportation use among Queen’s University employees pre- versus post-COVID-19 pandemic. Methods: Self-reported, repeated cross-sectional, and quasi-experimental data were used from the Surveys of Commute Patterns among Queen’s University Employees, conducted in 2013 to 2017 (n = 3874) and 2022 (n = 2430). Respondents’ primary mode of transportation between home and workplace was categorized as either AT, passive transportation (PT), or mixed transportation (MT) when different modes were combined. A descriptive analysis and chi-square test were performed to compare the levels of transportation use before and after the COVID-19 pandemic. A series of logistic regressions were conducted to identify the individual- and microsystem-level correlates of AT use before and after the COVID-19 pandemic. Results: AT use decreased after the COVID-19 pandemic, from 28.3% to 23.6%, mirroring the increased use of passive transport (52.1% to 55.4%). Within-subject comparisons showed that young and low-income respondents were the most likely to shift transport types from pre- to post-COVID-19 pandemic. At all time points, females/women were less likely to use AT than males/men. After the COVID-19 pandemic, income emerged as a key correlate of AT use. Conclusion: Significant world events like the COVID-19 pandemic appear to influence transportation choices and modes in unique ways among Queen’s employees. In general, AT was found to decline. Age and household income were important correlates for commute shifting since the COVID-19 pandemic. Sex/gender became a more important correlate in shaping transport mode post-COVID-19. To encourage shifting to more sustainable and health-enhancing transport modes among those with low and/or declining rates of AT, changes to supportive social environment and infrastructure must be considered in the Queen’s University community.