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Osteopontin stimulates invasion of NCI‐h295 cells but is not associated with survival in adrenocortical carcinoma
Copyright policy )Osteopontin stimulates invasion of NCI‐h295 cells but is not associated with survival in adrenocortical carcinoma
Abstract Gene array studies indicated that osteopontin (OPN) mRNA is highly expressed in adrenocortical carcinomas (ACCs). OPN enhances invasiveness, proliferation, and metastasis formation, and is associated with poor survival in some malignant diseases. Integrin αvβ3 has been shown to mediate OPN effects on invasion. In this study, we demonstrated OPN and integrin αvβ3 expression in normal adrenal glands and benign adenomas, with staining seen exclusively in adrenocortical cells as well as even stronger staining in ACC. Western blot analysis confirmed overexpression of OPN in ACC (p < 0.01). With Matrigel invasion assays, we have shown that OPN greatly stimulates the invasiveness of NCI‐h295 cells (>six‐fold increase, p < 0.001). Transfection with integrin αvβ3 further increased invasiveness after OPN stimulation (p < 0.001). This increase was reversed by the addition of an anti‐integrin β3 antibody, indicating a functional relationship of OPN and integrin αvβ3 in ACC. With tissue arrays, we confirmed high OPN expression in 147 ACC samples. However, no association with survival was seen in Kaplan‐Meier analysis including 111 patients with primary tumours graded for OPN staining and follow‐up data available. In conclusion, our in vitro data indicate that OPN and integrin αvβ3 may act as a functional complex facilitating the invasiveness of adrenocortical tumours. This relationship remains of relevance to our understanding of carcinogenesis, but further studies are needed to address the physiological and pathological function of OPN in adrenal tissue. Copyright © 2009 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
- University Hospital Würzburg Germany
- University Health Network Canada
- Universität Hamburg Germany
- Mount Sinai Hospital United States
- University of Würzburg Germany
Microsoft Academic Graph classification: Pathology medicine.medical_specialty Biology Metastasis medicine Adrenocortical carcinoma Osteopontin Integrin alphaVbeta3 Tissue microarray Adrenal cortex medicine.disease Blot medicine.anatomical_structure Cancer research biology.protein Immunohistochemistry
Medical Subject Headings: stomatognathic system
Adenoma, Blotting, Western, Kaplan-Meier Estimate, Transfection, Statistics, Nonparametric, Pathology and Forensic Medicine, Cell Line, Tumor, Adrenal Glands, Adrenocortical Carcinoma, Humans, Neoplasm Invasiveness, Oligonucleotide Array Sequence Analysis, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, Integrin alphaVbeta3, Immunohistochemistry, Adrenal Cortex Neoplasms, Osteopontin
Adenoma, Blotting, Western, Kaplan-Meier Estimate, Transfection, Statistics, Nonparametric, Pathology and Forensic Medicine, Cell Line, Tumor, Adrenal Glands, Adrenocortical Carcinoma, Humans, Neoplasm Invasiveness, Oligonucleotide Array Sequence Analysis, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, Integrin alphaVbeta3, Immunohistochemistry, Adrenal Cortex Neoplasms, Osteopontin
Microsoft Academic Graph classification: Pathology medicine.medical_specialty Biology Metastasis medicine Adrenocortical carcinoma Osteopontin Integrin alphaVbeta3 Tissue microarray Adrenal cortex medicine.disease Blot medicine.anatomical_structure Cancer research biology.protein Immunohistochemistry
Medical Subject Headings: stomatognathic system
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).13 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10% citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).13 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!- University Hospital Würzburg Germany
- University Health Network Canada
- Universität Hamburg Germany
- Mount Sinai Hospital United States
- University of Würzburg Germany
- University of Greifswald Germany
- University of Leeds United Kingdom
Abstract Gene array studies indicated that osteopontin (OPN) mRNA is highly expressed in adrenocortical carcinomas (ACCs). OPN enhances invasiveness, proliferation, and metastasis formation, and is associated with poor survival in some malignant diseases. Integrin αvβ3 has been shown to mediate OPN effects on invasion. In this study, we demonstrated OPN and integrin αvβ3 expression in normal adrenal glands and benign adenomas, with staining seen exclusively in adrenocortical cells as well as even stronger staining in ACC. Western blot analysis confirmed overexpression of OPN in ACC (p < 0.01). With Matrigel invasion assays, we have shown that OPN greatly stimulates the invasiveness of NCI‐h295 cells (>six‐fold increase, p < 0.001). Transfection with integrin αvβ3 further increased invasiveness after OPN stimulation (p < 0.001). This increase was reversed by the addition of an anti‐integrin β3 antibody, indicating a functional relationship of OPN and integrin αvβ3 in ACC. With tissue arrays, we confirmed high OPN expression in 147 ACC samples. However, no association with survival was seen in Kaplan‐Meier analysis including 111 patients with primary tumours graded for OPN staining and follow‐up data available. In conclusion, our in vitro data indicate that OPN and integrin αvβ3 may act as a functional complex facilitating the invasiveness of adrenocortical tumours. This relationship remains of relevance to our understanding of carcinogenesis, but further studies are needed to address the physiological and pathological function of OPN in adrenal tissue. Copyright © 2009 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.