publication . Article . 2004

Urine protein profiling with surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry

Stefan Schaub; John A. Wilkins; Tracey Weiler; Kevin Sangster; David N. Rush; Peter Nickerson;
Open Access
  • Published: 01 Jan 2004 Journal: Kidney International, volume 65, issue 1, pages 323-332 (issn: 0085-2538, Copyright policy)
  • Publisher: Elsevier BV
Abstract
Urine protein profiling with surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry. Background In the last few years there has been an increasing interest in exploring the human proteome. In particular, efforts have focused on developing strategies to generate reproducible protein maps of normal cells, tissues, and biologic fluids, from which studies can then compare protein expression between different groups (e.g., healthy individuals vs. those with a specific pathologic state). Methods Various extrinsic factors (instrument settings, matrix composition, urine storage post void, freeze-thaw cycles) and intrinsic factors (blood in urine, ...
Persistent Identifiers
Subjects
Fields of Science and Technology classification: 03 medical and health sciences0302 clinical medicine0303 health sciences030220 oncology & carcinogenesis030304 developmental biology
free text keywords: Nephrology, proteomics, urine protein profiling, SELDI-TOF-MS, mass spectrometry, Mass spectrometry, Urine, Ion suppression in liquid chromatography–mass spectrometry, Surface-enhanced laser desorption/ionization, Reproducibility, Chemistry, Time-of-flight mass spectrometry, Proteomics, Human proteome project, Chromatography
Funded by
CIHR
Project
  • Funder: Canadian Institutes of Health Research (CIHR)
,
SNSF| Proteomic-basierende Identifikation von Biomarkern für akute und chronische Nierentransplantatabstossung.
Project
  • Funder: Swiss National Science Foundation (SNSF)
  • Project Code: 68421
  • Funding stream: Careers | Fellowships | Fellowships for prospective researchers
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