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Prunus domestica Pathogenesis-Related Protein-5 Activates the Defense Response Pathway and Enhances the Resistance to Fungal Infection

Authors: Ashraf El-kereamy; Islam El-sharkawy; Rengasamy Ramamoorthy; Ali Taheri; Deena Errampalli; Prakash Kumar; Subramanian Jayasankar;

Prunus domestica Pathogenesis-Related Protein-5 Activates the Defense Response Pathway and Enhances the Resistance to Fungal Infection

Abstract

Pathogenesis-related protein-5 (PR-5) has been implicated in plant disease resistance and its antifungal activity has been demonstrated in some fruit species. However, their roles, especially their interactions with the other defense responses in plant cells, are still not fully understood. In this study, we have cloned and characterized a new PR-5 cDNA named PdPR5-1 from the European plum (Prunus domestica). Expression of PdPR5-1 was studied in different cultivars varying in resistance to the brown rot disease caused by the necrotrophic fungus Monilinia fructicola. In addition transgenic Arabidopsis, ectopically expressing PdPR5-1 was used to study its role in other plant defense responses after fungal infection. We show that the resistant cultivars exhibited much higher levels of transcripts than the susceptible cultivars during fruit ripening. However, significant rise in the transcript levels after infection with M. fructicola was observed in the susceptible cultivars too. Transgenic Arabidopsis plants exhibited more resistance to Alternaria brassicicola. Further, there was a significant increase in the transcripts of genes involved in the phenylpropanoid biosynthesis pathway such as phenylalanine ammonia-lyase (PAL) and phytoalexin (camalexin) pathway leading to an increase in camalexin content after fungal infection. Our results show that PdPR5-1 gene, in addition to its anti-fungal properties, has a possible role in activating other defense pathways, including phytoalexin production.

Subjects by Vocabulary

Library of Congress Subject Headings: lcsh:Medicine lcsh:Science lcsh:R lcsh:Q

Microsoft Academic Graph classification: Plant defense against herbivory Camalexin Arabidopsis thaliana chemistry.chemical_classification biology Phytoalexin Monilinia fructicola Plant disease resistance Microbiology Botany Pathogenesis-related protein Alternaria brassicicola biology.organism_classification chemistry

Keywords

Environmental Impacts, Indoles, Agricultural Biotechnology, Arabidopsis, Gene Identification and Analysis, Plant Science, Gene Expression Regulation, Plant, Molecular Cell Biology, Promoter Regions, Genetic, Plant Proteins, Multidisciplinary, Genetically Modified Organisms, Q, R, Alternaria, Agriculture, Plants, Genetically Modified, Medicine, Prunus, Agrochemicals, Signal Transduction, Research Article, Biotechnology, Marker-Assisted Selection, Science, Arabidopsis Thaliana, Molecular Sequence Data, Plant Pathogens, Crops, Mycology, Microbiology, Fruits, Molecular Genetics, Agricultural Production, Model Organisms, Plant and Algal Models, Genetics, Amino Acid Sequence, RNA, Messenger, Biology, Transgenic Plants, Plant Diseases, Internet, Base Sequence, Computational Biology, Crop Diseases, Plant Pathology, Immunity, Innate, Sustainable Agriculture, Thiazoles, Plant Biotechnology, Sequence Alignment

60 references, page 1 of 6

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  • citations
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    Top 10%
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    Top 10%
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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
80
Top 10%
Top 10%
Top 10%
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